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Human c5b 9 elisa kit

Manufactured by BD

The Human C5b-9 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of C5b-9 levels in human serum or plasma samples. It utilizes antibodies specific for the C5b-9 complex to quantify its concentration.

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3 protocols using human c5b 9 elisa kit

1

Cytokine and Complement Analysis in NHP and Mice

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Analysis of pro-inflammatory cytokines plasma levels was conducted using NHP and mouse-specific multiplex assay (K15056D and K15048D, respectively; Meso Scale Diagnostics, Rockville, MD). In addition, cynomolgus monkey MCP-1 and TNFα were analyzed using single analyte assay kits (K156UCK, K156NND, respectively; Meso Scale Diagnostics). Similarly, mouse MCP-1 was analyzed using single-plex assay kit (K152NN, Meso Scale Diagnostics).
Mouse C3 complement component levels were analyzed using Mouse Complement C3 ELISA Kit (ab157711, Abcam, Waltham, MA). C5b9 levels in cynomolgus monkey plasma were analyzed using Human C5b9 ELISA Kit (558315, BD, Franklin Lakes, NJ).
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2

Quantifying Complement and Chemokine Factors

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The Human C5b-9 ELISA Kit, Human C3ac ELISA Kit, and Human C5a ELISA Kit (BD OptEIA) were used to determine C5b-9 (membrane attack complex), C3a, and C5a complement components, respectively. The Human CXCL12/SDF-1 ELISA Kit (R&D Systems) was used to determine SDF-1 [40 (link),43 (link),46 (link),47 (link)].
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3

Quantifying Complement and Chemokine Levels

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C5b-9 (MAC), C3a and C5a complement components were determined by using the Human C5b-9 ELISA Kit, Human C3a ELISA Kit and Human C5a ELISA Kit (BD OptEIA), respectively.
SDF-1 was determined using a Human CXCL12/SDF-1 ELISA Kit (R&D Systems) [58 (link), 62 (link)–64 (link)].
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