All materials were purchased from commercial suppliers and used without further purification, unless otherwise noted. Cy5.5-NHS was purchased from GE Healthcare (Piscataway, NJ, USA). Succinimidyl iodoacetate (SIA), 2-iminothiolane hydrochloride (Traut's reagent), albumin from bovine serum (BSA), and Tf were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methoxy-PEG5000-b-PCL15000 was purchased from Daigang Biomaterial Co., Ltd. (Jinan, Shandong, China). NH2-PEG5800-b-PCL19000 was purchased from Polymer Source Inc. (Montreal, Quebec, Canada). Other chemicals and reagents used in the study were of analytical grade.
All cell lines were preserved in our lab. BALB/c nude mice (male, 4 to 6 weeks) were purchased from Hunan Slake Jingda Experimental Animal Co. Ltd., Changsha, China (Animal Qualification Certification No. 43004700000595). All animal studies were approved by the Animal Experimentation Ethics Committee of College of Life Science and Technology, Huazhong University of Science and Technology and carried out in compliance with the guidelines approved by the Science and Technology Department of Hubei Province.
All cell lines were preserved in our lab. BALB/c nude mice (male, 4 to 6 weeks) were purchased from Hunan Slake Jingda Experimental Animal Co. Ltd., Changsha, China (Animal Qualification Certification No. 43004700000595). All animal studies were approved by the Animal Experimentation Ethics Committee of College of Life Science and Technology, Huazhong University of Science and Technology and carried out in compliance with the guidelines approved by the Science and Technology Department of Hubei Province.