Lps derived from escherichia coli serotype 0111 b4

SRG3^β-actin B6 and SRG3^CD2 B6 mice were provided by Dr. Rho Hyun Seong (Seoul National University, Seoul, Korea). CD1d KO mice were provided by Dr. A. Bendelac (University of Chicago, IL, USA). SRG3^β-actin B6 mice were further crossed with CD1d KO mice to obtain CD1d KO/SRG3^β-actin B6 mice. All mice used in this study were on a B6 genetic background, maintained at Sejong University, and used for experiments at 6–12 weeks of age. Mice were maintained on a 12 h light/12 h dark cycle in a temperature-controlled barrier facility with free access to food and water. Mice were fed a γ-irradiated sterile diet and provided with autoclaved tap water. Age- and sex-matched mice were used for all experiments. The animal experiments were approved by the Institutional Animal Care and Use Committee at Sejong University (SJ-20161102, 11-15-2016). LPS derived from Escherichia coli (serotype 0111:B4) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

C57BL/6 (B6) wild type (WT) mice were purchased from Jung Ang Lab Animal, Inc. (Seoul, Korea). The IL4/GFP (4get) and IFNγ/YFP (yeti) cytokine reporter mice were kindly provided by Dr. R. Locksley (University of California at San Francisco, CA, USA). Foxp3/GFP reporter B6 mice and CD11c-diphtheria toxin receptor (DTR) transgenic (tg) mice were obtained from Dr. Rho H. Seong and Dr. E. Choi, respectively (Seoul National University, Seoul, Korea). All of the mice were on a B6 genetic background, were maintained at Sejong University, and were used for experiments at 6–12 weeks of age. They were maintained on a 12-h light/12-h dark cycle in a temperature-controlled barrier facility with free access to food and water. These mice were fed with a γ-irradiated sterile diet and autoclaved tap water. In this study, age- and sex-matched mice were used for all the experiments. The animal experiments were approved by the Institutional Animal Care and Use Committee at Sejong University (SJ-20130802). Our experiments were conducted in a blinded and randomized trial. For the induction of colitis, DSS with a molecular weight of 36–50 kDa was purchased from MP Biomedicals (Solon, OH, USA). LPS derived from Escherichia coli (serotype 0111:B4) was purchased from Sigma-Aldrich (St. Louis, MO, USA).