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2 protocols using pe labeled anti mouse cd124

1

Phenotyping of In Vitro Differentiated BM-MDRCs

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The in vitro differentiated BM-MDRC were prepared for flow cytometry by first incubating in FACS staining buffer (PBS + 3% FBS) containing 2.0 μg/ml of the mAb 2.4G2 (BD Pharmingen, Franklin Lakes, NJ) at 4°C for 30 minutes. These cells were then stained with several fluorochrome conjugated anti-mouse monoclonal antibodies for cellular phenotyping as follows: PE- labeled anti-mouse Gr-1 (clone: RB6-8C5), PerCPcy5.5-labeled anti-mouse Ly-6C (clone: HK1.4), FITC-labeled anti-mouse Ly-6G (clone: 1A8), APC-labeled anti-mouse F4/80 (clone: BM8), PE- labeled anti-mouse PDL-1 (clone: M1H5), PECy-7-labeled anti-mouse CD11c (clone: N418), PE-labeled anti-mouse CD115 (clone: AFS98) (all these antibodies were obtained from eBiosciences, San Diego CA); APCCy-7 labeled anti-mouse CD11b (clone: M1/70) and PE-labeled anti-mouse CD124 (clone: mIL4-R-M1) from BD Biosciences, San Jose, CA; and PE-labeled anti-mouse iNOS (Santa Cruz Biotechnologies, Dallas, TX).
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2

Phenotyping of In Vitro Differentiated BM-MDRCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The in vitro differentiated BM-MDRC were prepared for flow cytometry by first incubating in FACS staining buffer (PBS + 3% FBS) containing 2.0 μg/ml of the mAb 2.4G2 (BD Pharmingen, Franklin Lakes, NJ) at 4°C for 30 minutes. These cells were then stained with several fluorochrome conjugated anti-mouse monoclonal antibodies for cellular phenotyping as follows: PE- labeled anti-mouse Gr-1 (clone: RB6-8C5), PerCPcy5.5-labeled anti-mouse Ly-6C (clone: HK1.4), FITC-labeled anti-mouse Ly-6G (clone: 1A8), APC-labeled anti-mouse F4/80 (clone: BM8), PE- labeled anti-mouse PDL-1 (clone: M1H5), PECy-7-labeled anti-mouse CD11c (clone: N418), PE-labeled anti-mouse CD115 (clone: AFS98) (all these antibodies were obtained from eBiosciences, San Diego CA); APCCy-7 labeled anti-mouse CD11b (clone: M1/70) and PE-labeled anti-mouse CD124 (clone: mIL4-R-M1) from BD Biosciences, San Jose, CA; and PE-labeled anti-mouse iNOS (Santa Cruz Biotechnologies, Dallas, TX).
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