Lc grade acetonitrile

Standards of ABE, RIB, and PAL were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Benzimidazole (BEN) was purchased from Sigma-Aldrich (Burlington, MA, USA). Tablets of ABE (Verzenios) and RIB (Kisqali), as well as capsules of PAL (Ibrance), which are clinically available, were used. Running buffers were prepared from 50 mM sodium phosphate solution with pH = 2.5 and 1 M sodium hydroxide solution, both purchased from Agilent Technologies (Santa Clara, CA, USA). LC-grade acetonitrile and methanol were purchased from VWR International (Radnor, PA, USA). Formic acid was purchased from Kemika (Zagreb, Croatia). Ultra-pure water used for the preparation of all solutions and running buffers was obtained using a Milli-Q system.

Citrinin (CIT), 1-hydroxy-2-naphthoic acid, 1,4-dihydroxy-2-naphthoic acid, 2,2-azobis isobutyronitrile (AIBN), trimethylolpropane trimethacrylate (TRIM), methacrylic acid (MAA), and PBS tablets were obtained from Sigma (Steinheim, Germany). Barium chloride, N,N-dimethylaminoethyl methacrylate (DAEM), ethylene glycol dimethacrylate (EGDMA), methacrylamide (MAM), salicylic acid, acetic acid, ammonia (25% aq.), sodium hydroxide solution (1M), orthophosphoric acid, and Tween 20 were purchased from Merck (Darmstadt, Germany). LC-grade acetonitrile and methanol were from VWR (Langenfeld, Germany), acetone was from Honeywell (Seelze, Germany). 2-Naphthoic acid was obtained from Acros Organics (Geel, Belgium), 2-oxocyclohexane carboxylic acid was obtained from Fluorochem (Karlsruhe, Germany), and rhodizonic acid dehydrate (RHO) was obtained from abcr (Karlsruhe, Germany). Ochratoxin A (OTA) was provided by HPC Standards (Borsdorf, Germany) and EASI-EXTRACT^® Citrinin columns were provided by R-Biopharm Rhone Ltd. (Glasgow, UK). Cereal products (wheat flour, pasta, rice, whole grain rice crispies, and oat flakes) were purchased from local markets and did not contain detectable levels of CIT. CIT stock and working solution were prepared according to DIN EN 17203 [38 (link)] and stored in acid-washed amber vials at −20 °C.

For LC/DAD analysis, LC-grade acetonitrile and methanol were purchased from VWR (Fontenay-Sous-Bois, France). Vanillic, protocatechuic, caffeic, ferulic, p-coumaric, sinapic, syringic, and chlorogenic acids, as well as enniatin A, enniatin A₁, enniatin B, and enniatin B₁ were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Reagents were purchased from Scharlau (Barcelona, Spain), Sigma Aldrich (Saint-Quentin-Fallavier, France) and Thermo Fischer Scientific (Illkirch, France).