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7 protocols using petroleum ether

1

Synthesis of Polyurethane-Based Composites

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Guanidine carbonate, ethyl acetoacetate, alpha-acetyl-gamma-butyrolactone, triethylamine, ethylene glycol monoallyl ether, 1-thioglycerol, 2,2-dimethoxy-2-phenylacetophenone, hexamethylene diisocyanate, polytetramethylene ether glycol (PTMEG, Mn = ∼1000 g mol−1), ditinbutyl dilaurate (DBTDL) were purchased from Aladdin Chemistry Regent Co., Ltd (China). Triethylene glycol bis(3-tert-butyl-4-hydroxy-5-methylphenyl)propionate (AO-70) was obtained from Beijing Additive Research Institute (China). Hydroxyl groups modified multi-walled carbon nanotubes (MWCNTs-OH) with hydroxyl content of 1.85 wt% was purchased from Chengdu Organic Chemicals Co. Ltd (China). 4,4′-Dicyclohexylmethane diisocyanate (HMDI) was kindly provided by Wanhua Chemical Group Co., Ltd (China). Absolute alcohol, sodium hydroxide, dichloromethane, hydrochloric acid, p-toluene sulfonic acid, cyclohexane, sodium bicarbonate, tetrahydrofuran, anhydrous sodium sulfate, acetone, petroleum ether and dimethyl formamide (DMF) were purchased from Chengdu Kelong Chemical Reagent Co. Ltd (China). DMF was dehydrated with activated 4A molecular sieves for at least one week before use, the other reagents were used as received.
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2

Extraction and Analysis of Tartary Buckwheat

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The Tartary buckwheat (Chuanqiao No. 2) was purchased from Liangshan Prefecture, China, while the TBB was provided and processed by the Huantai Biotechnology Co., Ltd. (Chengdu, China). The rutin standard, gallic acid standard (chromatography grade, purity ≥ 98%), Folin-phenol reagent, and DPPH were purchased from the Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). The copper sulfate (CuSO4), potassium sulfate (K2SO4), boric acid (H3BO3), sulfuric acid (H2SO4), ethanol, petroleum ether, hydrogen peroxide (H2O2), phosphate, methanol (analytical grade), and methyl red were obtained from the Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). The sodium hydroxide (NaOH), ferrous chloride (FeCl2), and salicylic acid were purchased from the Tianjin Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China), while the aluminum trichloride (AlCl3) and potassium acetate were acquired from the Guangdong Guanghua Technology Co., Ltd. (Shantou, China).
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3

Antimicrobial Evaluation of Natural Compounds

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Folin-Ciocalteu Reagent, chloramphenicol (Beijing Solarbio Co., Ltd., China); ellagic acid, galic acid monohydrate, caffeic acid, quercetin and theophyline (Shanghai Winherb Medical Science Co., Ltd., China); tannin acid (Tianjin Dongliqu Tianda Chemical Co., Ltd., China); ethanol, methanol, petroleum ether, ethyl acetate, n-butanol, dimethyl sulfoxide (DMSO) (Chengdu Kelong Chemical Co., Ltd., China); sodium carbonate (Na2CO3), sodium borohydride (NaBH4), aluminum chloride (AlCl3), hydrochloric acid, acetic acid, chloranil, vanillin, phosphomolybdic acid, sodium tungstate, and tetrahydrofuran (THF) (Tianjin Bodi Chemical Co., Ltd., China); chromatographic grade methanol and acetonitrile (Sigma-Aldrich Co., St. Louis, USA).
Muller Hilton Agar (MHA) and nutrient broth (NB) (Qingdao Hope Bio- Technology Co., Ltd., China).
S. aureus, Listeria monocytogenes (L. monocytogenes), S. paratyphi A, Salmonella typhimurium (S. typhimurium) and Salmonella, L. enteritidis (S. enteritidis) (Microbial Culture Collection Center of Guangdong Institute of Microbiology, China).
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4

Graphene-Reinforced Polymer Microspheres

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Styrene (St) and Span 80 were obtained from Innochem Technology Co., Ltd., Beijing, China. Divinylbenzene (DVB), sodium persulfate (Na2S2O8) and sodium dodecyl sulfonate (SDS) were obtained from Aldrich, Shanghai, China. Graphene (GN, lamellae size 5–15 µm) was supplied by Deyang Carbonene Technology Co., Ltd., and the lamellae were composed of five to six layers of graphene. Absolute ethanol, petroleum ether, chloroform, toluene and acetone were all purchased from Chengdu Kelong Chemical Reagent Factory. Ultrapure deionized water from an ultrapure water machine was used to prepare all solutions.
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5

Biomimetic Bone Scaffold Fabrication

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PLA 4032D (Mw = 2.1×105 g/mol, PDI = 1.7, 98% L-isomer content) was obtained from NatureWorks. Tetracalcium phosphate (TTCP) was provided by Ensail Beijing Co., Ltd. (China). D-Lactide was purchased from Jinan Daigang Biomaterial Co., Ltd (China). Methylbenzene, dichloromethane (DCM) and petroleum ether were purchased from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). Stannous octanoate (Sn(Oct)2), and 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) were purchased from Aladdin Chemistry Co., Ltd (Shanghai, China). The mouse fibroblast cell (L929) was purchased from American Type Culture Collection (ATCC, USA.) Male Sprague-Dawley (SD) rats (1 week) were purchased from Chengdu Dossy Experimental Animals Co., Ltd (Chengdu, China). The Animal Ethics Committee of West China Hospital of Sichuan University granted permission for the experiments. All the animal procedures in the current study were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Sichuan University.
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6

Synthesis and Evaluation of Hydrophobic Carmofur Derivatives

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Sodium hydroxide, potassium hydroxide, dinitrile diamine, anhydrous acetone, petroleum ether, ethyl acetate, dichloromethane, anhydrous potassium carbonate, dimethyl sulfoxide, Tween 80, and hydrochloric acid p-toluenesulfonyl chloride were purchased from Chengdu Chron Chemicals Co. Ltd, China. Tetraethylene glycol monomethyl ether, 4-hydroxybenzonitrile, diethylene glycol methyl ether, and hydrophobic carmofur (HCFU) were purchased from Tansoole (Shanghai, China). The dialysis bag was purchased from Spectrum Laboratories, Inc. USA. DMEM culture medium, fetal bovine serum (FBS), and pancreatin were purchased from Cytiva HyClone Laboratories, USA. Paraformaldehyde was purchased from Tianjin ZhiYuan Reagent Co. Ltd, China. Hoechst 33342 cell was purchased from Merck Sigma-Aldrich, USA. Counting kit-8 (CCK-8) was purchased from Beyotime Biotech Inc., China.
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7

Extraction and Characterization of Microbial Compounds

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Citric acid, petroleum ether (boiling range 30 °C to 60 °C), and acetone were purchased from ChengDu Chron Chemicals Co., Ltd. (Chengdu, China). Nutrient agar (NA) and rose bengal agar (RBA) broth were purchased from Beijing Aoboxing Biological Technology Co., Ltd. (Beijing, China). All other chemicals were of analytical grade and were purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China).
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