Csu x1 head

Cells were seeded into 8-well dishes (Thistle Scientific, UK) to enable experiments to be performed in parallel. Before imaging, the culture medium was replaced with Leibovitz’s L-15 medium (Gibco Life Technologies, UK) supplemented with 10% foetal bovine serum and penicillin/streptomycin. Cells were imaged on a DeltaVision micrososcope equipped with an environmental chamber at 37°C (API, USA) with a QuantEM camera (Photometrics, USA) and Lambda LS illumination (Sutter, USA) as previously described ^{10 (link)}, or a spinning disc microscope (Intelligent Imaging Innovations, Colorado, USA) equipped with a CSU-X1 head (Yokogawa, Japan) and a QuantEM:512sc EMCCD camera (Photometrics, USA). In fig. 2e, 3d and fig.4, images of DIC and fluorescence were captured at 6 min intervals and the fluorescence intensities were measured and analysed using ImageJ/Fiji software as previously described ^{10 (link)}.