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Mv 122

Manufactured by Flow Tech
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The MV-122 is a versatile laboratory equipment designed for precise vacuum measurements. It features a compact and durable construction, making it suitable for a wide range of applications in various research and industrial settings.

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Lab products found in correlation

Microfil, by Flow Tech (1 mentions) Anti α smooth muscle actin antibody, by Agilent Technologies (1 mentions) Adenosine, by Merck Group (1 mentions) Papaverine, by Merck Group (1 mentions) Acetylcholine, by Merck Group (1 mentions) Sodium nitroprusside, by Merck Group (1 mentions) Dihydroethidium dhe, by Merck Group (1 mentions) Micro ct, by Bruker (1 mentions)

Close spelling variants of this product

Microfill MV‐122 Microfil MV-122 yellow Microfil MV-122

8 protocols using mv 122

1

Vascular Imaging of Murine Femurs and Kidneys

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Microfil, a radio-opaque silicone rubber compound containing lead chromate (MV-122; Flow Tech Inc., Boulder, CO), was used as previously described (13 (link)–15 (link)). Femurs and kidneys dissected from mice sacrificed at 5, 10, and 20 weeks of age (N = 5 for each age group) were fixed for 48 h in 10% buffered formalin to ensure complete tissue fixation. Angiographic images were obtained using plain radiography and μCT (12-μM isotropic voxel size) as previously described (12 (link)).
Cole H.A., Moore-Lotridge S.N., Hawley G.D., Jacobson R., Yuasa M., Gewin L., Nyman J.S., Flick M.J, & Schoenecker J.G. (2021). The Deleterious Effects of Impaired Fibrinolysis on Skeletal Development Are Dependent on Fibrin(ogen), but Independent of Interlukin-6. Frontiers in Cardiovascular Medicine, 8, 768338.
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2

Renal Microvascular Architecture Analysis

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Renal microvascular architecture was assessed using micro-CT, as previously described (Eirin et al., 2012 (link)). Kidney segments were perfused (Microfil MV122) with a radio-opaque silicone polymer (Flow Tech, Carver, MA) using a saline-filled cannula ligated within a segmental artery. Samples were prepared and scanned, and images analyzed using Analyze™ to calculate spatial density of cortical microvessels and microvascular tortuosity (Zhu et al., 2004 ). In addition, renal tissue sections were stained with a-smooth muscle actin (SMA, DakoCytomation A/S, Glostrup, Denmark) and media-to-lumen ratio calculated. Renal expression of hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and VEGF receptor (VEGFR)-2, was assessed by Western Blotting.
Eirin A., Ferguson C.M., Zhu X.Y., Saadiq I.M., Tang H., Lerman A, & Lerman L.O. (2020). Extracellular vesicles released by adipose tissue-derived mesenchymal stromal/stem cells from obese pigs fail to repair the injured kidney. Stem cell research, 47, 101877.
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3

Measuring Myocardial Microvasculature Using Micro-CT

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Myocardial microvascular architecture was assessed using a micro-CT scanner. The proximal left anterior descending artery was cannulated and perfused under physiological pressure with an intravascular contrast agent (MV-122, Flow Tech, Carver, MA, USA). A transmural section of the LV (2 cm3) was scanned and spatial density of small (<200 μm), medium (200 to 300 μm) and large (>300 μm) microvessels in the sub-epicardium and sub-endocardium calculated [21 (link), 36 (link)] using Analyze™. In addition, immunostaining with anti-α-smooth muscle actin (SMA) antibody (DakoCytomation, Glostrup, Denmark) was performed and media-to-lumen ratio calculated to assess microvascular wall thickening [22 (link)].
Eirin A., Zhu X.Y., Ferguson C.M., Riester S.M., van Wijnen A.J., Lerman A, & Lerman L.O. (2015). Intra-renal delivery of mesenchymal stem cells attenuates myocardial injury after reversal of hypertension in porcine renovascular disease. Stem Cell Research & Therapy, 6(1), 7.
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4

Microfil Perfusion for Mouse Vasculature

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After 42 days, mice were anesthetized and overdosed with sodium pentobarbital at a dose of 80mg/kg, i.p. A horizontal midline incision was made right below the diaphragm to avoid pneumothorax and to ensure blood flow during catheter insertion. The abdominal aorta was cannulated, the catheter secured with 5–0 suture and a 6mL heparinized (100U/mL) saline solution containing a mixture of vasodilators (10µM acetylcholine, 10µM adenosine, 100µM papaverine and 200µM sodium nitroprusside; Sigma-Aldrich, St. Louis, MO) was infused at 1 mL/min with a 3mL plastic syringe (Becton Dickson, Franklin Lakes, NJ) for blood washout. The inferior vena cava was severed to allow for drainage of blood and the infusing solution. For the Microfil perfusion, a curing agent (5% v/v) was added to catalyze the mixture of the components (compound and diluent mixed in equal amounts, according to the manufacturer recommendations; MV122 Flow-Tech Inc Carver, MA) with a working time of 20 minutes before the start of polymerization. 0.5mL of the prepared compound was drawn in a 1mL syringe and injected at 1mL/h with care taken to remove any perfusate that contaminated the surrounding tissue, skin or fur. Perfused specimens were placed at room temperature for 30 minutes and then stored at 4°C overnight to allow for complete polymerization of the contrast agent.
Adeyemo A., Johnson C., Stiene A., LaSance K., Qi Z., Lemen L, & Schultz J.E. (2020). Limb functional recovery is impaired in fibroblast growth factor-2 deficient (FGF2) mice despite chronic ischemia-induced vascular growth. Growth factors (Chur, Switzerland), 38(2), 75-93.
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5

Quantifying Vascular Changes in Bone Fractures

Cited 1 time
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Each animal was perfused with Microfil MV-122 (Flowtech, #MV-122), a lead chromate-based contrast agent, after which the vascular structure surrounding the fracture site in the tibia was examined by microCT with the following parameters: 55 kV, 145 μA, and a 300 ms integration time. 3D images were generated using Scanco software. In this experiment, we analyzed the center of the fracture area where the atrophic region was located and the surrounding region. Quantification of the vessel number was based on 20 slices centered on the fracture site as previously described.56 (link) The relative vessel count was calculated by normalization of the experimental group to the control group.
Wang C., Ying J., Nie X., Zhou T., Xiao D., Swarnkar G., Abu-Amer Y., Guan J, & Shen J. (2021). Targeting angiogenesis for fracture nonunion treatment in inflammatory disease. Bone Research, 9, 29.
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6

Quantifying Myocardial Oxidative Stress and Microvasculature

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Myocardial in situ production of superoxide anion was detected by dihydroethidium (DHE) staining (20μM/l; Sigma) [7 (link)], and myocardial capillary density was determined by CD31 staining (Bio-Rad, Oxford, UK). In addition, myocyte cross-sectional area and myocardial fibrosis were calculated in wheat germ agglutinin (WGA)- and trichrome-stained slides, respectively. All staining images were semi-automatically quantified in 15–20 fields and the results from all fields averaged [29 (link)]. Myocardial microvascular structure was assessed by micro-CT, as previously described [24 (link)]. The LV wall was perfused under physiological pressure with an intravascular contrast agent (MV-122; Flow Tech, Carver, MA) through a branch of the left circumflex coronary artery. A transmural portion (2 cm3) of the LV was scanned, and images were analyzed with Analyze™. Spatial density of microvessels (20-500μm) in the subepicardium and subendocardium and vessel tortuosity (an index of vessel immaturity) were calculated, as previously described [24 (link)].
Farahani R.A., Yu S., Ferguson C.M., Zhu X.Y., Tang H., Jordan K.L., Saadiq I.M., Herrmann S.M., Chade A.R., Lerman A., Lerman L.O, & Eirin A. (2021). Renal Revascularization Attenuates Myocardial Mitochondrial Damage and Improves Diastolic Function in Pigs with Metabolic Syndrome and Renovascular Hypertension. Journal of cardiovascular translational research, 15(1), 15-26.
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7

Microfil Kidney Perfusion Fixation

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Tissue preparation: We used methods similar to those employed in our previous study. 4 Nine male Sprague Dawley rats were anaesthetised with sodium pentobarbitone (60 mg/kg. i.p; Sigma-Aldrich, St Louis, MO, USA). A cannula was inserted retrogradely into the abdominal aorta and the kidneys were then cleared with phosphate buffer and perfusion fixed at physiological pressure with Karnovsky's fixative (4% paraformaldehyde and 4% glutaraldehyde in 0.2 M phosphate buffer). Microfil® (10-12 ml; MV-122; Flow Tech, Carver, MA, USA) was then infused into the kidney until the kidneys were uniform in colour and the Microfil® flowed freely from the veins. The renal artery and vein were then ligated. The kidneys were removed, decapsulated, and weighed, before being immersed in fixative, and transported immediately to the Imaging and Medical Therapy Beam Line (IMBL) at the Australian Synchrotron.
Ngo J.P., Le B., Khan Z., Kett M.M., Gardiner B.S., Smith D.W., Melhem M.M., Maksimenko A., Pearson J.T, & Evans R.G. (2017). Micro-computed tomographic analysis of the radial geometry of intrarenal artery-vein pairs in rats and rabbits: Comparison with light microscopy. Clinical and experimental pharmacology & physiology, 44(12).
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8

Coronary Microcirculation Imaging in Rats

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Rats were anesthetized with 2% isoflurane and underwent left common carotid artery catheterization with a 22 G Teflon tube, and the tube orifice reached the level of the thoracic aortic sinus. After humanely euthanizing the rat, the coronary arteries were perfused with a pre-configured MicroFil (Flow Tech Inc, MV-122) molded contrast medium until they fully filled the coronary arteries and cardiac capillaries, the common carotid artery was ligated to prevent contrast outflow, and the anesthetized rats were euthanized. Rat carcasses were maintained at 4 °C overnight to facilitate contrast cross-linking, and subsequently enhanced contrast scanning of the rat cardiac microcirculation was performed using a micro-CT (Bruker micro-CT).
Xue F., Zhao S., Tian H., Qin H., Li X., Jian Z., Du J., Li Y., Wang Y., Lin L., Liu C., Shang Y., He L., Xing M, & Zeng W. (2024). Two way workable microchanneled hydrogel suture to diagnose, treat and monitor the infarcted heart. Nature Communications, 15, 864.
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