Mn chloride tetrahydrate mncl2 4h2o

Chemical reagents were purchased from the following sources: Mn chloride tetrahydrate (MnCl₂·4H₂O) from Fisher Scientific (Pittsburgh, PA); Cu chloride (CuCl₂), calcium chloride (CaCl₂), Dextran-70, hydroxyethyl piperazineethanesulfonic acid (HEPES), monoclonal anti-mouse β-actin antibody, 2-mercaptoethanol, phenylmethylsulfonyl fluoride (PMSF), polyacrylamide and tetramethyl-ethylenediamine (TEMED) from Sigma Chemicals (St Louis, MO); ultrapure nitric acid from VWR international (Chicago, IL); Fluor Alexa-488 conjugated secondary antibody from Life Technologies (Carlsbad, CA); protease inhibitor cocktail from Calbiochem (San Diego, CA); Tris base, glycine, sodium dodecyl sulfate (SDS), 2xLaemmli sample buffer, Triton X-100, and clarity Western ECL substrate from Bio-Rad (Hercules, CA); Aβ₄₀ pure PTD human protein and Aβ₄₀ human ELISA kit from Invitrogen (Waltham, MA); anti-RAGE antibody and anti-LRP1 antibody from Abcam (Cambridge, MA); Anti-Aβ₄₀ antibody from Biolegend (San Diego, CA); rat LRP1/CD91 ELISA Kit and rat AGER/RAGE ELISA Kit from LifeSpan BioSciences (Seattle WA); Radioactive ¹⁴C-sucrose (specific activity: 495 mCi/mmol) from Moravek Biochemicals (Brea, CA); and Eco-lite-(+) scintillation cocktail from MP Biomedicals (Irvine, CA). All reagents were of analytical grade, HPLC grade, or the best available pharmaceutical grade.

Chemical reagents were purchased from the following sources: Mn chloride tetrahydrate (MnCl₂•4H₂O) from Fisher Scientific (Pittsburgh, PA); Mn and Cu standard solutions were from SCP Science (Champlain, NY); ketamine from Fort Dodge (Fort Dodge, IA ); and xylazine from Vedco (St. Joseph, MO). All reagents were analytical grade, HPLC grade, or the best available pharmaceutical grade.