CPA was purchased from LKT Laboratories (St Paul, MN). 177LuCl3 was purchased from Perkin Elmer (Waltham, MA). Tritium-labeled CPA ([3H]CPA; 1.0 mCi/mg CPA) was obtained from Moravek Biochemicals (Brea, CA). Lecithin E80, lecithin S100, and oleic acid were purchased from Lipoid (Ludwigshafen, Germany). 1-(4-Isothiocyanatobenzyl) diethylenetriaminepentaacetic acid (DTPA-bz-SCN) was obtained from Macrocyclics (Dallas, TX). (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) was purchased from Life Technologies (Carlsbad, CA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO) or Fisher Scientific (Pittsburgh, PA).
4T1 murine breast cancer cells and Miapaca-2 human pancreatic ductal adenocarcinoma cells were purchased from American Type Culture Collection (Manassas, VA) and maintained in Dulbecco modified Eagle medium supplemented with 10% fetal bovine serum and 1% antibiotics. Both cell lines have activated Sonic Hedgehog pathway [10 (link), 23 (link), 24 (link)].
4T1 murine breast cancer cells and Miapaca-2 human pancreatic ductal adenocarcinoma cells were purchased from American Type Culture Collection (Manassas, VA) and maintained in Dulbecco modified Eagle medium supplemented with 10% fetal bovine serum and 1% antibiotics. Both cell lines have activated Sonic Hedgehog pathway [10 (link), 23 (link), 24 (link)].