For western blotting, anti-Flag (M2) (F3165–5MG, 1:5000 dilution), anti-α-tubulin (T6199–200UL, 1:5000 dilution) and anti-β-actin (A5441–100UL, 1:5000 dilution) monoclonal antibodies were obtained from Sigma-Aldrich. An anti-Myc (sc-40, 1:500 dilution) antibody was purchased from Santa Cruz Biotechnology. An anti-hemagglutinin (HA) monoclonal antibody (MMS-101P, 1:3000 dilution) was obtained from Covance. An anti-STRN4 (A304–573A-T, 1:1000 dilution) was obtained from Bethyl laboratories. Anti-phospho-YAP (S127) (4911S, 1:1000 dilution), anti-phospho-LATS1 (T1079) (8654S, 1:1000 dilution) and anti-LATS1 (3477S, 1:1000 dilution) antibodies were purchased from Cell Signaling Technology. The YAP polyclonal antibody was raised against a GST-YAP fusion protein and the antisera were affinity-purified using an AminoLink Plus Immobilization and Purification Kit (Pierce) (Wang et al., 2011 (link)).
For immunostaining, an anti-YAP (sc-101199, 1:200 dilution) monoclonal antibody was purchased from Santa Cruz Biotechnology. An anti-HA polyclonal antibody (3724S, 1:3000 dilution) was obtained from Cell Signaling Technology.
For immunohistochemical staining, an anti-YAP (14074S, 1:20 dilution) monoclonal antibody was purchased from Cell Signaling Technology. An anti-STRN4 (A304–573A-T, 1:20 dilution) was obtained from Bethyl laboratories.
For immunostaining, an anti-YAP (sc-101199, 1:200 dilution) monoclonal antibody was purchased from Santa Cruz Biotechnology. An anti-HA polyclonal antibody (3724S, 1:3000 dilution) was obtained from Cell Signaling Technology.
For immunohistochemical staining, an anti-YAP (14074S, 1:20 dilution) monoclonal antibody was purchased from Cell Signaling Technology. An anti-STRN4 (A304–573A-T, 1:20 dilution) was obtained from Bethyl laboratories.