MamBo cell lines were established from mammary tumors of FVBhuHER2 virgin female mice. Tumors were minced and set in culture with an appropriate medium as previously described [22 (link)]. Cell lines were stabilized and cultured in DMEM (Thermo Fisher Scientific, Monza, Italy) that was supplemented with 20% fetal bovine serum (FBS, Thermo Fisher Scientific), 30 µg/ml bovine pituitary extract (Corning Life Sciences, Glendale, AZ, USA) and 0.5% v/v MITO Serum Extender (Corning). Cell cultures were maintained at 37 °C in a humified 5% CO2 atmosphere.
Bovine pituitary extract
Manufactured by Corning
Sourced in United States
Bovine pituitary extract is a biological material derived from the pituitary gland of bovine (cattle) origin. It contains a complex mixture of hormones, enzymes, and other biologically active substances naturally found in the pituitary gland. The core function of bovine pituitary extract is to serve as a source of these natural components for use in various laboratory applications and research studies.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Mito serum extender, by Corning (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hcc1954, by American Type Culture Collection (1 mentions)
Mda mb 453, by American Type Culture Collection (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using bovine pituitary extract
1
Establishment and Culture of MamBo Cell Lines
2
Culturing Mammary Carcinoma Cell Lines
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Cell line MAMBO89, established from a mammary carcinoma of huHER2 transgenic mouse,38 (link) was cultured in DMEM (Thermo Fisher Scientific) additioned with 20% fetal bovine serum (FBS, Thermo Fisher Scientific), 30 µg/ml bovine pituitary extract (Corning) and 5 µl/ml MITO+ SERUM EXTENDER (Corning). MAMBO89 cell line was monitored for murine origin by PCR and for full-length HER2 transgene expression by PCR and flow cytometry. Human ovarian cancer cell line SK-OV-3 and human breast cancer cell lines MDA-MB-453, BT-474 and SKBR3 (all from American Type Culture Collection, ATCC) were kindly given by Dr. Serenella M. Pupa (Istituto Nazionale dei Tumori, Milan, Italy). SK-OV-3, MDA-MB-453 and BT-474, were authenticated by DNA fingerprinting on the 11th November 2010 (performed by DSMZ, Braunschweig, Germany). HCC1954, MDA-MB-231 and BT-474 C5 cell lines were purchased from ATCC. Cells were routinely cultured in RPMI medium (Thermo Fisher Scientific) supplemented with 10% FBS. Human rhabdomyosarcoma cell line SJ-RH4, lacking HER2 expression, was cultured in DMEM additioned with 10% FBS. Cell lines were maintained at 37°C in a humidified 5% CO2 atmosphere, except SJ-RH4, which was kept at 7% CO2.
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