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Cyto id hypoxia oxidative stress detection kit

Manufactured by Enzo Life Sciences
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Sourced in United States

The Cyto-ID Hypoxia/Oxidative Stress Detection Kit is a fluorescent staining solution used to detect the presence of hypoxia and oxidative stress in live cells. It provides a quantitative method to measure these cellular conditions.

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Lab products found in correlation

Anti rabbit cy2 secondary antibody, by Jackson ImmunoResearch (1 mentions) Hypoxia red dye, by Enzo Life Sciences (1 mentions) Cell culture media and supplements, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Cyto-ID (44 citations) Cyto-ID kit (7 citations) Hypoxia/oxidative stress detection kit (5 citations) Hypoxia Detection Kit (3 citations) Hypoxia/oxidative detection kit (2 citations)

2 protocols using cyto id hypoxia oxidative stress detection kit

1

Assaying Peritoneal Leukocyte Hypoxia

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Mouse peritonitis was induced by zymA (Sigma-Aldrich, St Louis, MO, USA), peritoneal exudate leucocytes were collected at 6, 12, 24 and 48 h after zymA administration and peritoneal leucocytes without zymA stimulated were also collected (0 h). The cells were recovered by centrifugation at 300g for 5 min, resuspended in 200 μl of Hypoxia/Oxidative Stress Detection Mix (5 × 105 cells per sample) and then incubated under normal tissue culture conditions for 0.5 h. Samples were washed and analysed using flow cytometry according to product manual (Cyto-ID Hypoxia/Oxidative Stress Detection Kit, Enzo Life Sciences, ENZ-51042, Farmingdale, NY, USA).
Luo B., Wang J., Liu Z., Shen Z., Shi R., Liu Y.Q., Liu Y., Jiang M., Wu Y, & Zhang Z. (2016). Phagocyte respiratory burst activates macrophage erythropoietin signalling to promote acute inflammation resolution. Nature Communications, 7, 12177.
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2

Hypoxia Detection in Cell Cultures

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Cell culture media and supplements were purchased from Invitrogen. Mouse HIF1α primary antibody for Western blot was purchased from Novus Biologicals, and rabbit β-tubulin primary antibody was purchased from Cell Signaling. Rabbit MAP2 primary antibody for immunocytochemistry was purchased from Abcam, and antirabbit Cy2 secondary antibody was purchased from the Jackson Immunoresearch Laboratory. Hypoxia Red dye was purchased as part of the CytoID Hypoxia/Oxidative Stress Detection Kit manufactured by Enzo Life Sciences (ENZ-51042). Other reagents were purchased from Sigma-Aldrich, unless otherwise specified.
Lizama-Manibusan B.N., Klein S., McKenzie J.R., Cliffel D.E, & McLaughlin B. (2016). Analysis of a Nitroreductase-Based Hypoxia Sensor in Primary Neuronal Cultures. ACS chemical neuroscience, 7(9), 1188-1191.
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