Pcdna3 ha mob1

Manufactured by Addgene

PcDNA3-HA-MOB1 is a plasmid vector that expresses the MOB1 protein with a hemagglutinin (HA) tag. MOB1 is a regulatory protein involved in various cellular processes. This plasmid can be used for the expression and study of the MOB1 protein in mammalian cell lines.

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Lab products found in correlation

Pcmv myc mst1, by Addgene (2 mentions) Pcmv2 flag sav1, by Addgene (2 mentions) P2xflag cmv2 lats1, by Addgene (2 mentions) 8xgtiic luciferase, by Addgene (1 mentions) Pqcxih myc yap, by Addgene (1 mentions)

Spelling variants of this product

PcDNA3.1 (119 citations) PcDNA3-HA (3 citations)

2 protocols using pcdna3 ha mob1

Plasmids for Cell Signaling Studies

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Plasmids pCEFL-HA, pCEFL-HA-GαqQL, pCEFL-HA-Gαq-DREADD, pCEFL-3x-Flag-Renilla-luciferase were described previously (Marinissen et al., 2003 (link); Teramoto et al., 2003 (link)). pCEFL-myr-FAK was described previously (Chikumi et al., 2002 (link); Igishi et al., 1999 (link)). Plasmids pCMV-myc-MST1 (Addgene #8847, originally from Joseph Avruch’s lab), pCMV2-FLAG-SAV1 (Addgene #18970, originally from Marius Sudol’ lab), pcDNA3-HA-MOB1 (Addgene #32835, originally from Kunliang Guan’s lab), p2xFLAG-CMV2-LATS1 (Addgene #18971, originally from Marius Sudol’s lab) and 8xGTIIC-luciferase (Addgene #34615, originally from Stefano Piccolo’s Lab).

Feng X., Arang N., Cosimo Rigiracciolo D., Sang Lee J., Yeerna H., Wang Z., Lubrano S., Kishore A., Pachter J.A., Maggiolini M., Kostenis E., Schlaepfer D.D., Tamayo P., Chen Q., Ruppin E, & Gutkind J.S. (2019). A Platform of Synthetic Lethal Gene Interaction Networks Reveals that the GNAQ Uveal Melanoma Oncogene Controls the Hippo Pathway through FAK. Cancer cell, 35(3), 457-472.e5.

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Plasmid Constructs for YAP-LATS1 Signaling

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Plasmids pCMV-myc-MST1 (Addgene #8847 from Joseph Avruch’s lab)^{57 (link)}, pCMV2-FLAG-SAV1 (Addgene #18970 from Marius Sudol’s lab)^{58 (link)}, pcDNA3-HA-MOB1 (Addgene #32835 from Kunliang Guan’s lab)^{14 (link)}, p2xFLAG-CMV2-LATS1 (Addgene #18971 from Marius Sudol’s lab)^{58 (link)}, and pQCXIH-Myc-YAP (Addgene #33091 from Kunliang Guan’s lab)^{14 (link)} were used. GFP-expressing vector was used as control.

Ando T., Arang N., Wang Z., Costea D.E., Feng X., Goto Y., Izumi H., Gilardi M., Ando K, & Gutkind J.S. (2021). EGFR Regulates the Hippo pathway by promoting the tyrosine phosphorylation of MOB1. Communications Biology, 4, 1237.

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