For co-localization studies, 5·104 per cm2 HEK293 cells were seeded on EZ-multiwell slides (Millipore, Burlington, MA, USA). Cells were transfected using equimolar quantities of pEGFP-PARP1 (a gift from A. Chiarugi) and pRFP-E2F1 (a gift from B. Su) and synchronized by double thymidine treatment. Upon release from cell cycle block, cells were fixed with 2% paraformaldehyde (pH 7.4), nuclei were counterstained using DAPI (Invitrogen, Waltham, MA, USA), and fluorescent images from three independent experiments were obtained using a Leica TCS SP2 microscope.
Ez multiwell slides
Manufactured by Merck Group
Sourced in United States
The EZ-multiwell slides are a versatile laboratory tool designed for various applications. They feature multiple wells on a single slide, allowing for efficient sample handling and processing. The core function of these slides is to provide a convenient platform for conducting experiments or assays that require the use of multiple samples or replicates.
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Lab products found in correlation
2 protocols using ez multiwell slides
1
Co-localization of PARP1 and E2F1
2
Co-localization Study of PARP1 and E2F1
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For co-localization studies, 5 × 104 per cm2 HEK293 cells were seeded on EZ-multiwell slides (Millipore, Burlington, MA, USA). Cells were transfected using equimolar quantities of pEGFP-PARP1 (a gift from A. Chiarugi) and pRFP-E2F1 (a gift from B. Su) and synchronized by double thymidine treatment. Upon release from cell cycle block, cells were fixed with 2% paraformaldehyde (pH 7.4) at various times. Nuclei were stained using DAPI (Invitrogen, Waltham, MA, USA) and fluorescent images from three different experiments were taken using a Leica TCS SP2 microscope.
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