Anti flag pa1 984b

Manufactured by Thermo Fisher Scientific

The Anti-FLAG (PA1-984B) is a laboratory reagent used for the detection and purification of proteins tagged with the FLAG epitope. It is a highly specific monoclonal antibody that recognizes the eight-amino acid FLAG peptide sequence. This product can be used in various applications, including Western blotting, immunoprecipitation, and protein purification.

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Lab products found in correlation

Nocodazole, by Merck Group (1 mentions) Aphidicolin, by Merck Group (1 mentions) Alexa fluor 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Mab810r, by Merck Group (1 mentions) Anti tubulin dm1a, by Merck Group (1 mentions) Alexa fluor 488 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Anti flag m2, by Merck Group (1 mentions) Ab9485, by Abcam (1 mentions) Anti cgas, by Cell Signaling Technology (1 mentions) Anti irf3, by Cell Signaling Technology (1 mentions) Anti tbk1, by Cell Signaling Technology (1 mentions) Pe anti mouse cd62l, by BioLegend (1 mentions) Streptavidin agarose, by Merck Group (1 mentions) Anti phospho irf3, by Cell Signaling Technology (1 mentions) Apc anti mouse perforin, by BioLegend (1 mentions) Pe anti mouse ifn γ, by BioLegend (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Anti phospho tbk1, by Cell Signaling Technology (1 mentions) Fitc anti mouse cd11c, by BioLegend (1 mentions) 2 3 cgamp, by InvivoGen (1 mentions)

2 protocols using anti flag pa1 984b

Immunofluorescence and Western Blotting Protocol

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Where indicated in the figure legends and on the figures, aphidicolin (2 μg/ml; Sigma) or nocodazole (50 ng/ml; Sigma) was diluted in fresh medium and added to cells. The following antibodies were from commercial sources: anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (ab9485; Abcam), anti-tubulin (DM 1A; Sigma), anti-FLAG (PA1-984B, Thermo), anti-FLAG (M2; Sigma), anti-HA (HA.11, BioLegend), anti-cytomegalovirus for IE1 (ab30924; Abcam), and anti-cytomegalovirus for IE1 and IE2 (MAB810R; Millipore). Monoclonal antibodies against IE1 (1B12) and IE2 (3H9) were described previously (70 (link)). Infrared (IR) dye 680- and 800-conjugated secondary antibodies (Li-Cor) were used for Western blotting. Alexa Fluor 488-conjugated secondary antibody (catalog no. A-11017; Invitrogen) and Alexa Fluor 594-conjugated secondary antibody (catalog no. A-11020; Invitrogen) were used for immunofluorescence.

Lyon S.M., Yetming K.D., Paulus C., Nevels M, & Kalejta R.F. (2020). Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain. mBio, 11(5), e02410-20.

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Comprehensive Antibody Characterization Protocol

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The following antibodies were used: anti-cGAS (#31659), anti-cGAS (#83623), anti-TBK1 (#3013), anti-phospho-TBK1 (#5483), anti-IRF3 (#4302), anti- phospho-IRF3 (#4947), anti-Myc (#2276) were purchased from Cell Signaling Technology; anti-VSV-G (V5507), anti-MB21D2 (SAB2108874) were purchased from Sigma-Aldrich; anti-Flag (PA1-984B) was purchased from Thermo Fisher Scientific; anti-HA (sc-7392), anti-Actin (sc-58673) were purchased from Santa Cruz Biotechnology; FITC anti-mouse CD3 (#100203), PE anti-mouse IFN-γ (#505807), APC anti-mouse Perforin (#154403), PE/Cy7-anti-mouse CD80 (#104733), PerpCP/Cyanine5.5 anti-mouse CD86 (#105027), FITC anti-mouse CD11c (#117305) were purchased from BioLegend; PE anti-mouse CD62L (#561918), PerCP-Cy5.5 anti-mouse CD8α (#561109), APC anti-mouse CD69 (#560689), PE/Cyanine7 anti-human/mouse Granzyme B (#372213) were purchased from BD Biosciences; APC anti-mouse-MHC class II (ab93559) was purchased from Abcam. All antibodies were used according to the manufacturer’s instructions. 2′, 3′-cGAMP was purchased from InvivoGen. For Biotin Pull-Down assay, Streptavidin-Agarose (S1638, Sigma-Aldrich) was used.

Liu H., Yan Z., Zhu D., Xu H., Liu F., Chen T., Zhang H., Zheng Y., Liu B., Zhang L., Zhao W, & Gao C. (2023). CD-NTase family member MB21D2 promotes cGAS-mediated antiviral and antitumor immunity. Cell Death and Differentiation, 30(4), 992-1004.

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