The supernatant was isolated from each culture condition and were kept at − 80 °C until further analysis. After thawing, the supernatants were used immediately for cytokine quantification using following kits according to the manufacturer’s instructions: Proteome Profiler Human Cytokine Array Kit for 36 cytokines (Biotechne, ARY005B). Secreted interleukins were identified using the following ELISA kits (sample dilution 1:5000): TNF-α (Invitrogen, BMS223HS), IL-6 (Invitrogen, BM213HS), IL-8 (Invitrogen, BMS204-3INST), IL-1β (Invitrogen, BMS224HS) and IL-10 (Invitrogen, BMS215HS). Quantification was analyzed performed by a microplate reader, TECAN M1000 pro (TECAN, Switzerland), software i-control™ (version: 2.0.10.0). The statistical analysis of the data was performed in GraphPad Prism (v8.0.2).
Bms215hs
Manufactured by Thermo Fisher Scientific
The BMS215HS is a laboratory equipment designed for general use. It features adjustable settings and specifications to accommodate a variety of applications.
Automatically generated - may contain errors
2 protocols using bms215hs
1
Cytokine Quantification in Cell Culture
2
Inflammation Profiling of WJMSC on LiCS
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For the inflammation assay, WJMSC were cultured on the LiCS scaffold with mesenchymal stem cell medium for 1 day, the cell was lysis by NP40 and collected protein for analyzed the inflammation-related marker. The quantity of TNF-α (BMS223-4, Invitrogen), IL-1β (#BMS224-2, Invitrogen), and IL-10 (#BMS215HS, Invitrogen) was estimated by enzyme-linked immunosorbent assay according to the manufacturer's instructions. The concentrations of targeted protein in each sample were considered by comparing the absorbance with a standard curve.
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