L 1 tosylamido 2 phenylenylethyl chloromethyl ketone tpck

Rabbit polyclonal antibody specific for phosphate-p85, a heterodimer of phosphatidylinositol 3 kinase (PI3K), p-Akt, p-IKK were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibodies specific for PI3K, Akt, IKK, NF-κB, β-actin, and mouse polyclonal antibodies specific for OSM were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The recombinant human adiponectin was purchased from PeproTech (Rocky Hill, NJ, USA). PI3K inhibitors (Wortmannin and Ly294002), Akt inhibitor, and NF-κB inhibitors pyrrolidine dithiocarbamate (PDTC) and L-1-tosylamido-2-phenylenylethyl chloromethyl ketone (TPCK) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Small-interfering RNAs (siRNAs) against p85, Akt, and p65 were purchased from Dharmacon Research (Lafayette, CO, USA). OSM ELISA kit was purchased from R&D Systems (Minneapolis, MN, USA). DMEM, fetal bovine serum (FBS), and all the other cell culture reagents were purchased from Gibco life technologies (Grand Island, NY, USA).

Rabbit polyclonal antibody specific to phosphate p-PI3K was obtained from Cell Signaling Technology (Danvers, MA); rabbit polyclonal antibodies specific to αvβ3, p-FAK, FAK, c-Src, PI3K, p-p65, p65, β-actin, and mouse polyclonal antibodies specific to p-c-Src and OSM from Santa Cruz Biotechnology (Santa Cruz, CA). Human recombinant CCN1 was obtained from PeproTech (Rocky Hill, NJ), FAK inhibitor (FAKi) and c-Src inhibitor (PP2), PI3K inhibitors (Wortmannin and Ly294002), NF-κB inhibitors pyrrolidine dithiocarbamate (PDTC) and L-1-tosylamido-2-phenylenylethyl chloromethyl ketone (TPCK) from Sigma-Aldrich (St. Louis, MO). NF-κB luciferase kit was purchased from Stratagene (La Jolla, CA). DMEM, fetal bovine serum (FBS), all other cell culture reagents from Gibco-BRL Life Technologies (Grand Island, NY).