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2 protocols using malondialdehyde bis diethyl acetal

1

Antioxidant Assays for α-Cypermethrin and Curcumin

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α-Cypermethrin (CAS # 52315-07-8; purity ≥97%) was a kind gift from Nanjing Essence Fine-Chemical Co., Ltd. (Nanjing, China). Curcumin (LKT-C8069; CAS # 458-37-7; purity >90%) was from LKT Laboratories Inc. (St. Paul, MN, USA). Malondialdehyde bis (diethyl acetal) was from Merck KGaA (Darmstadt, Germany); 2,4-dinitrophenylhydrazine (DNPH), catalase (CAT; product no. C30), Red cell lysing Buffer Hybri-Max™ (product no. R7757), potassium periodate, iodonitrotetrazolium chloride, superoxide dismutase from bovine erythrocytes, xanthine, xanthine oxidase, and Purpald® were from Sigma–Aldrich Chemie GmbH (Steinheim, Germany). Hydrogen peroxide solution (35%) was purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany).
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2

Antioxidant assays using DTNB and MDA

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The chemicals 5,5′-dithiobis-2-nitrobenzoic acid (DTNB), gallic acid, glutathione (reduced and oxidized), glutathione reductase (GR), 1-methyl-2-vinylpyridinium trifluoromethanesulfonate (M2VP) and β-nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt (NADPH) were all products of Sigma–Aldrich (South Africa). Formaldehyde (histological grade), lipopolysaccharide (LPS; Escherichia coli, serotype 0111:B4), perchloric acid (PCA), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), 2-thiobarbituric acid (TBA) and trichloroacetic acid (TCA) were purchased from Sigma–Aldrich Chemicals, (St Louis, MO, USA). Ascorbic acid and malondialdehyde bis (diethyl acetal) (MDA) were products from Merck (Johannesburg, South Africa). Red palm oil (CarotinoTM baking fat) was a gift from Carotino SDN BHD (Johar-Bahru, Malaysia). All other reagents used were of analytical grade.
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