Echomri 3 in 1 instrument

Manufactured by Echo Medical Systems

Sourced in United States

The EchoMRI 3-in-1 instrument is a medical device developed by Echo Medical Systems. It is designed to perform three distinct analysis functions: magnetic resonance imaging (MRI), spectroscopy, and diffusion imaging. The core function of this instrument is to provide comprehensive imaging and analysis capabilities for medical research and clinical applications.

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Lab products found in correlation

Thermalert th 5 thermometer, by Physitemp (1 mentions) Mouse hemoglobin a1c hba1c assay kit, by Crystal Chem (1 mentions) Accu chek performa, by Roche (1 mentions) Anti fas antibody, by Abcam (1 mentions) Anti actin antibody, by Merck Group (1 mentions) Quantikine elisa, by R&D Systems (1 mentions)

Close spelling variants of this product

EchoMRI 3-in-1 model instrument EchoMRI 3-in-1 EchoMRI 3-1 EchoMRI instrument The 3-in-1 EchoMRI

3 protocols using echomri 3 in 1 instrument

Analyzing Mouse Body Composition and Lipid Excretion

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Body composition was analysed in mice treated with Nnmt and control ASO for 5 weeks using an EchoMRI 3-in-1 instrument (Echo Medical Systems). To measure body composition after fasting, food was removed from Nnmt-ASO- or control-ASO-treated mice for 16h. Body temperature was measured intrarectally using a Thermalert TH-5 thermometer (Physitemp). To analyse faecal lipid excretion, lipid content of faeces was extracted using chloroform:methanol (2:1) and air-dried under a fume hood.

Kraus D., Yang Q., Kong D., Banks A.S., Zhang L., Rodgers J.T., Pirinen E., Pulinilkunnil T.C., Gong F., Wang Y.C., Cen Y., Sauve A.A., Asara J.M., Peroni O.D., Monia B.P., Bhanot S., Alhonen L., Puigserver P, & Kahn B.B. (2014). Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature, 508(7495), 258-262.

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Glycemia and Body Composition Analysis

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Body weight and food intake were recorded weekly. After an overnight fast, glycemia was measured weekly using a drop of whole blood taken from the tail and an Accu-Chek Performa blood glucose meter (Roche, Mannheim, Germany). After 6 weeks of treatment, fat and lean mass were recorded by MRI (EchoMRI 3-in-1 instrument; Echo Medical Systems, Houston, TX, USA). Finally, animals were fasted overnight (16 h) and euthanized by cervical dislocation. Whole blood was harvested right after the death of the mice and a small portion was collected in an EDTA-treated tube and put on ice until analysis. Glycated hemoglobin (HbA1c) levels were measured in EDTA-treated whole blood with a mouse Hemoglobin A1c (HbA1c) Assay Kit (Crystal Chem, Elk Grove Village, IL, USA) by comparing absorbance at 700 nm in a spectrometer with Hba1c controls according to the instructions of the manufacturer.

Haguet Q., Le Joubioux F., Chavanelle V., Groult H., Schoonjans N., Langhi C., Michaux A., Otero Y.F., Boisseau N., Peltier S.L., Sirvent P, & Maugard T. (2023). Inhibitory Potential of α-Amylase, α-Glucosidase, and Pancreatic Lipase by a Formulation of Five Plant Extracts: TOTUM-63. International Journal of Molecular Sciences, 24(4), 3652.

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Metabolic Profiling in Fasted Mice

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Serum triglycerides, cholesterol, free fatty acids (FFA) and glucose were assayed in mice following a 4 h fast. Triglyceride and cholesterol levels were determined using reagents from Thermo Scientific. FFA levels were assayed using reagents provided by Wako Chemicals. Serum glucose was measured using reagents from Sigma. G-CSF was measured using a Quantikine ELISA from R&D Systems. Bacterial endotoxin was measured using a limulus amebocyte lysate assay (Wei et al. 2012 (link)). Body composition was determined using an EchoMRI 3-in-1 instrument (Echo Medical Systems). Western blotting was performed using a rabbit polyclonal anti-FAS antibody from Abcam, an anti-actin antibody from Sigma, and antibodies against CHOP and Grp78 from Cell Signaling.

Lodhi I.J., Wei X., Yin L., Feng C., Adak S., Abou-Ezzi G., Hsu F.F., Link D.C, & Semenkovich C.F. (2015). Peroxisomal lipid synthesis regulates inflammation by sustaining neutrophil membrane phospholipid composition and viability. Cell metabolism, 21(1), 51-64.

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