Apc conjugated anti cd44
APC-conjugated anti-CD44 is a monoclonal antibody that binds to the CD44 cell surface antigen. CD44 is a transmembrane glycoprotein involved in cell-cell interactions, cell adhesion, and migration. The APC (Allophycocyanin) fluorescent label allows for detection and analysis of CD44-expressing cells using flow cytometry.
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13 protocols using apc conjugated anti cd44
Stemness Marker Analysis of PANC-1 Cells
Stem Cell Marker Profiling by Flow Cytometry
Characterization of Cancer Stem Cells
For apoptosis analysis, cells were stained with Annexin V-FITC Apoptosis Kit (K101, Biovision, Milpitas, CA, USA) according to the manufacturer’s instructions.
After the indicated labeling, cells were analyzed with MoFlo Astrios (Beckman-Coulter, CA, USA).
Immunoblotting and Flow Cytometry Antibodies
Thymocyte Apoptosis Profiling by TUNEL and Annexin V
Isolation of CD44+, CD133+ Cells
Comprehensive Stemness and EMT Marker Profiling
Stemness and Apoptosis Analysis of Pancreatic Cancer Cells
For analysis of stemness markers, cells (1×106) were labeled with PE‐conjugated CD24 (BD PharMingen™, cat. no. 555428) and APC‐conjugated anti‐CD44 (BD PharMingen™, cat. no. 559942), incubated at 4℃ in the dark for 30 min. Cells were then centrifuged and resuspended in PBS at a concentration of 1×108/ml, and subsequently sorted on a flow cytometer (BD Accuri C6 Plus; BD Biosciences).
For apoptosis and necrosis analysis, cells were cultured for 12 h prior to being treated with gem for a further 24 h. For flow cytometry analysis, cells were detached and labeled using an Annexin V‐PE/7‐AAD Apoptosis Detection kit (Nanjing KeyGen Biotech Co., Ltd.; cat. no. KGA1017) according to the manufacturer's protocol. Apoptotic and necrotic cell subsets were quantified using flow cytometry. A total of 2×104 cells were analyzed per sample. Annexin V‐PE−/7‐AAD− cells were considered as viable, Annexin V‐PE+/7‐AAD− cells were considered as early apoptotic, and Annexin V‐PE+/7‐AAD+ cells were considered as late apoptotic and necrotic cell populations.
Quantifying CD133 and CD44 in Cell Populations
ZIKV E Antigen-Stimulated Splenocyte Response
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