Jsm 5200

SEM analysis (SEM; JSM-5200, JEOL, Tokyo, Japan) was performed for Ag-doped CNT and both untreated (control) and treated specimens with a working distance of 10 mm, magnification of 2000× and 5000×, resolution of 3 nm, and an accelerating voltage of 30 kV. An additional SEM micrograph was taken for the powder with the magnification 20,000×.
Chemical analysis for the powder and the specimens was carried out, employing an environmental scanning electron microscope (SEM; JSM-5200, JEOL, Tokyo, Japan) and energy-dispersive X-ray spectroscopy (EDX; Oxford Inca Energy 350, Oxford Instruments, Abingdon, UK), with a working distance of 10 mm, resolution of 3 nm, and an accelerating voltage of 30 kV. The automatic identification of elements and element quantification in both wt.% and atomic % were performed following the collection of EDX spectra. SEM images and associated EDX spectra were carefully recorded.

SEM imaging was performed with a JEOL JSM-5200 (Tokyo, Japan) scanning electron microscope at 1 kV acceleration voltage on uncoated samples. The SEM images were collected on neat, non-sputter-coated, samples, whereas the fiber sizes, d, were calculated based on 50 measurements on three SEM images taken at different parts of the samples. The open-source ImageJ (version 1.52a, National Institutes of Health, Bethesda, MD, USA) software was used to carry out the measurements. Since the generated fibers do not follow a normal distribution, instead of average size and standard deviation, box plots were used. These provide more accurate information regarding the distribution while also taking outliers into account. To quantify the fiber dimensions, the median values given by the box plots were used.

For the determination of glandular trichome density from the upper epidermis of the leaf, a scanning electron microscope (SEM) was used (JEOL JSM-5200, Japan). The SEM was utilized in order to achieve small magnification, high contrast micrographs using secondary electrons at 20 mm working distance, with 1 of 5 kV accelerating voltage as function of sample charging. The fresh leaves of A. annua were collected at the full vegetation stage in July and were immediately subjected to analysis with the SEM. For the analysis 10 leaves of 10 randomly selected plants from each block were collected (a total of 80 plants sampled and 400 leaves/treatment analyzed). This represented almost completely the total upper leaves numbers of the plants. The youngest terminal leaf, of about 5 cm in length, on each plant was removed for sampling. The leaf surface was analyzed by starting with a fragment from the leaf mounted on a scanning surface in the form of a square with 0.6 cm sides (Figure 2). The number of glandular trichomes were counted with the multi-point tool, while the scanned leaf surface was measured with the polygon selection tool. Then the leaf surface was determined and the number of trichomes reported and averaged in mm².