Vt1200

Manufactured by Leica

Sourced in Germany, United States, United Kingdom, Japan, Australia, Canada, Israel, Switzerland

The VT1200S is a vibrating microtome designed for precision sectioning of biological samples. It features a high-precision feed system and a stable base for consistent, uniform sectioning.

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Lab products found in correlation

Lsm 880, by Zeiss (26 mentions) Lsm 710, by Zeiss (15 mentions) Bx51wi, by Olympus (14 mentions) Vt1000, by Leica (14 mentions) Lsm 780, by Zeiss (12 mentions) Multiclamp 700b amplifier, by Molecular Devices (10 mentions) Fd rapid golgistain kit, by FD NeuroTechnologies (9 mentions) Vibratome, by Leica (9 mentions) Lsm 780 confocal microscope, by Zeiss (9 mentions) Cacl2, by Merck Group (8 mentions) Lsm 700, by Zeiss (8 mentions) D glucose, by Merck Group (8 mentions) Tcs sp8, by Leica (8 mentions) Triton x 100, by Merck Group (8 mentions) Nahco3, by Merck Group (7 mentions) Multiclamp 700b, by Molecular Devices (7 mentions) Glutamax, by Thermo Fisher Scientific (7 mentions) Clampfit 10, by Molecular Devices (7 mentions) Paraformaldehyde (pfa), by Merck Group (7 mentions) Avertin, by Merck Group (6 mentions)

Spelling variants of this product

VT1200S vibratome (369 citations) VT1200S microtome (24 citations) VT1200S vibrating microtome (12 citations) VT1200 vibrating blade microtome (9 citations) VT1200 vibrating microtome (5 citations) VT1200s microslicer (4 citations) VT1200S slicer (3 citations) VT1200S tissue slicer (2 citations) VT1200S vibrotome (2 citations) VT1200 specimen holder (2 citations)

1 569 protocols using vt1200

Lignification and GUS Localization in Poplar

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Hybrid poplar 717 and three independent PtPL1-18 overexpression lines were propagated by tissue culture and transferred to soil. Plant height and stem diameter were measured after 4 months of growth, from six randomly selected individual plants per line. Cross sections were made from the 12th internodes and the corresponding petioles using vibratome (Leica VT 1200S, Germany). The sections were stained with 5% (w/v) phloroglucinol in 12% (v/v) HCl for indication of lignification, and observed under bright field microscope (Olympus BX63, Japan) for digital imaging.
For GUS assay, internode 4, 7, and 12 of the main stem and their corresponding petioles were collected from transgenic plants carrying PtPL1-18pro:GUS, washed twice with 50 mM sodium phosphate buffer (pH 7.0), and incubated in X-gluc reaction solution (1 mM X-gluc, 50 mM sodium phosphate, pH 7.0, 0.1% Triton X-100, 1 mM potassium ferricyanide and 1 mM potassium ferrocyanide) at 37°C overnight. Then 40 μm thick cross sections were prepared using vibratome (Leica VT 1200S, Germany), cleared with 75% ethanol and observed under bright-field microscope (Olympus BX63, Japan).

Bai Y., Wu D., Liu F., Li Y., Chen P., Lu M, & Zheng B. (2017). Characterization and Functional Analysis of the Poplar Pectate Lyase-Like Gene PtPL1-18 Reveal Its Role in the Development of Vascular Tissues. Frontiers in Plant Science, 8, 1123.

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Preparation of Medial Prefrontal Cortex Slices

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KCC2^+/− and WT littermate mice aged P35–45 were anesthetized with isoflurane and rapidly decapitated. Brains were quickly removed and placed into ice-cold ACSF solution (composition in mM: 124 NaCl, 4 KCl, 26 NaHCO₃, 1.26 NaH₂PO₄, 6 MgSO₄, 1 CaCl₂). A vibratome (Leica VT1200) was used to generate 300um thick medial prefrontal cortex (mPFC) coronal sections containing the anterior cingulate cortex (ACC) and prelimbic cortex (PrL). Slices were transferred to an oxygenated (95% oxygen, 5% CO2) holding chamber containing bicarbonate buffered ACSF at room temperature (composition in mM: 124 NaCl, 4 KCl, 26 NaHCO₃, 1.26 NaH₂PO₄, 3 MgSO₄, 2 CaCl₂).
For the KCC2 inhibitor (VU0240511, Delpire et al., 2009 (link)) experiments, brains were removed from WT mice as described above, but then placed into ice-cold, modified NMDG-containing ACSF solution (composition in mM: 110 NMDG, 110 HCl, 3 KCl, 10 MgCl₂ 6H₂0, 1.1 NaH₂PO₄ H₂0, 0.5 CaCl₂ dihydrate, 25 glucose, 3 pyruvic acid, 10 ascorbic acid, 25 NaHCO3). A vibratome (Leica VT1200S) was used to generate 300um thick mPFC coronal sections containing the ACC and PrL. Slices were allowed to recover in oxygenated (95% oxygen, 5% CO2) NMDG-based ACSF for 30 minutes at 34°C. Slices were then transferred to an oxygenated holding chamber at room temperature containing bicarbonate buffered ACSF as per above.

Anacker A.M., Moran J.T., Santarelli S., Forsberg C.G., Rogers T.D., Stanwood G.D., Hall B.J., Delpire E., Veenstra-VanderWeele J, & Saxe M.D. (2019). Enhanced social dominance and altered neuronal excitability in the prefrontal cortex of male KCC2b mutant mice. Autism research : official journal of the International Society for Autism Research, 12(5), 732-743.

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Preparation of Prefrontal Cortex and Hippocampus Slices

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Three- to four-week-old adult mice were deeply anesthetized with pentobarbital sodium before rapid decapitation and removal of the entire brain into ice-cold, oxygenated cutting solution (220 mM sucrose, 3 mM KCl, 5 mM MgCl₂, 1 mM CaCl₂, 1.25 mM NaH₂PO₄, 26 mM NaHCO₃, 10 mM glucose, 315 mOsm/l, 95% O₂/5% CO₂ and pH 7.3). Coronal slices (200 μm) of the prefrontal cortex (bregma 3.6–2.5 mm) were cut using a vibrating microtome (VT1200S; Leica), and then incubated in artificial cerebral spinal fluid (ACSF) (125 mM NaCl, 2.5 mM KCl, 2.5 mM CaCl₂, 1.5 mM MgSO₄, 1 mM NaH₂PO₄, 26.2 mM NaHCO₃, 11 mM glucose, 305 mOsm/L and pH 7.3) for 1 h at 34 °C. Slices were stored at room temperature until experimental use.
For hippocampus slices, entire brains were placed into ice-cold, oxygenated cutting solution (130.05 mM NaCl, 4.96 mM KCl, 0.30 mM MgSO₄, 11.02 mM MgCl₂ * 6H₂O, 20.01 mM HEPES, 1.5 mM CaCl₂, 22.02 mM NaHCO₃, 5.50 mM glucose, pH 7.3 adjusted by NaOH, 350–360 mOsm/l, 95% O₂/5% CO₂). The brain was then blocked for sectioning. Coronal slices (300–400 μm thick) were cut using a Vibratome (VT1200S, Leica), and incubated in ACSF (109.99 mM NaCl, 2.50 mM KCl, 2.50 mM CaCl₂, 1.50 mM MgSO₄, 1 mM KH₂PO₄, 26.19 mM NaHCO₃, 11 mM glucose, 300–310 mOsm/L and pH 7.3) for 1 h at 34 °C. Slices were stored at room temperature until experimental use.

Wang K., Lu J.M., Xing Z.H., Zhao Q.R., Hu L.Q., Xue L., Zhang J, & Mei Y.A. (2017). Effect of 1.8 GHz radiofrequency electromagnetic radiation on novel object associative recognition memory in mice. Scientific Reports, 7, 44521.

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Hippocampal Slice Preparation and Electrophysiology

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For patch clamp electrophysiology and oscillation experiments, adult mice (>P60) were anesthetized using 4% isoflurane followed by decapitation, and the brains were extracted in warm (30–35°C) sucrose solution (40 mM NaCl, 3 mM KCl, 7.4 mM MgSO₄.7H₂O, 150 mM sucrose, 1 mM CaCl₂, 1.25 mM NaH₂PO₄, 25 mM NaHCO₃, and 15 mM glucose; osmolality 300 ± 10 mOsmol/kg). Horizontal hippocampal slices (350 μm thick) were cut using a vibratome (Leica VT1200S) and were placed in an interface chamber containing warm artificial cerebrospinal fluid (aCSF) (126 mM NaCl, 3.5 mM KCl, 2 mM MgSO₄.7H₂O, 1.25 mM NaH₂PO₄, 24 mM NaHCO₃, 2 mM CaCl₂, and 10 mM glucose; osmolality 300 ± 10 mOsmol/kg). For the fEPSP experiments, adult mice were sacrificed by cervical dislocation followed by decapitation and brains were extracted in ice-cold dissecting solution [87 mM NaCl, 25 mM NaHCO₃, 10 mM glucose, 75 mM sucrose, 2.5 mM KCl, 1.25 mM NaH₂PO₄, 0.5 mM CaCl₂, and 7 mM MgCl₂]. Transverse hippocampal slices (300 μm thick) were cut using a vibratome (Leica, VT-1200S) and stored in dissecting solution at 35°C for 30 min and then in aCSF at 22°C for the duration of the experiment. All solutions were bubbled with carbogen gas [95% O₂/ 5% CO₂].

Kontou G., Antonoudiou P., Podpolny M., Szulc B.R., Arancibia-Carcamo I.L., Higgs N.F., Lopez-Domenech G., Salinas P.C., Mann E.O, & Kittler J.T. (2021). Miro1-dependent mitochondrial dynamics in parvalbumin interneurons. eLife, 10, e65215.

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Blade Vibration Characterization of Tissue Slicers

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To characterize the blade vibration output vs input of MEGAtome, two laser displacement sensors (ZX2; Omron) were used to measure the displacement of M1 and M2. The sensors were mounted to the stationary ground of the machine; they were configured to emit laser beams perpendicular to the surfaces being measured. The sensors' resolution is 1.5 μm, the measurement range is ±5 mm. Same sensor was used to measure the real blade vibration output of Leica VT1200. To characterize the blade out-of-plane displacement vs in-plane displacement of MEGA-Slicer and Leica VT1200, three high-precision capacitance probes (CPL290; Lion Precision) were used to measure out-of-plane displacement of the blade at three different locations, including the left edge, right edge, and the center of the blade. The noncontact probes detect small displacement by sensing changes of the capacitance of the electric field between the probe and the conductive object surface. The resolution of the sensors is 5 nm RMS. At the same time, a laser displacement sensor was used to measure the in-plane blade vibration. Measurements for both machines were performed with the same 80 mm-long blade (D554G50; Sturkey).

Park J., Wang J., Guan W., Gjesteby L., Pollack D., Kamentsky L., Evans N.B., Stirman J., Gu X., Zhao C., Marx S., Kim M.E., Choi S.W., Snyder M., Chavez D., Su-Arcaro C., Tian Y., Park C.S., Zhang Q., Yun D.H., Moukheiber M., Feng G., Yang X.W., Keene C.D., Hof P.R., Ghosh S., Frosch M.P., Brattain L.J., & Chung K. (2022). Integrated platform for multi-scale molecular imaging and phenotyping of the human brain.

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Preparation of Horizontal Mouse Brain Slices

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Mice were anesthetized with Euthasol-III (0.2 ml/kg, Med-Pharmex Inc., Pomona, CA, USA) and decapitated. Brains were quickly removed and placed in ice-cold (<4°C) sucrose solution containing the following reagents (in mM): NaCl 87, sucrose 75, KCl 2.5, CaCl₂ 1, MgCl₂ 7, NaH₂PO₄ 1.25, NaHCO₃ 25, glucose 25, which was aerated with 95% O₂ and 5% CO₂, pH 7.4. Horizontal cortical slices at 300 μm thickness were cut using Leica VT1200S (Leica Microsystems Inc., Buffalo Grove, IL, USA). Slices were then incubated for 40 min at 36°C before being maintained at room temperature until recording. Slices were submerged in a recording chamber filled with oxygenated artificial cerebrospinal fluid (ACSF, in mM): NaCl 124, KCl 2.5, CaCl₂ 2, MgCL₂ 1, NaH₂PO₄ 1.25, glucose 10, NaHCO₃ 26, pH 7.4.

Yang S.S., Li Y.C., Coley A.A., Chamberlin L.A., Yu P, & Gao W.J. (2018). Cell-Type Specific Development of the Hyperpolarization-Activated Current, Ih, in Prefrontal Cortical Neurons. Frontiers in Synaptic Neuroscience, 10, 7.

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Transcardiac Perfusion and Brain Sectioning

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Male C57Bl6/J mice (Taconic M&B, Denmark) were anesthetized with an intraperitoneal injection of 0.25 ml pentobarbital sodium (Apoteksbolaget, Sweden). Transcardiac perfusion was performed using phosphate-buffered saline (PBS) (137.0 mM NaCl, 2.70 mM KCl, 8.10 mM Na2HPO4) followed by 4% formaldehyde (HistoLab, Sweden) fixation. The brains were dissected and stored in 4% formaldehyde at 4°C overnight. The next day, the brains were washed twice in Tris-buffered saline (TBS) (0.04 M Trizma HCl, 0.01 M Trizma base, 0.15 M NaCl, pH 7.4) followed by 4% agarose gel (VWR, Germany) embedding. 70 μm coronal brain sections were cut with a vibratome Leica VT 1200 S (Leica Microsystems, Germany).

Ceder M.M., Lekholm E., Hellsten S.V., Perland E, & Fredriksson R. (2017). The Neuronal and Peripheral Expressed Membrane-Bound UNC93A Respond to Nutrient Availability in Mice. Frontiers in Molecular Neuroscience, 10, 351.

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Rice Pollen Development Staging

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The developmental stages of the rice pollen were defined in the same methods described in Itoh et al. [30 (link)] and Fujita et al. [14 (link)], based on anther length. Spikelets of different developmental stages were collected, fixed in PFA, and dehydrated through an ethanol series. The samples were embedded in Technovit 7100 resin (Hereaus Kulzer) and polymerized at room temperature. Transverse sections of 3 µm were cut using a Leica VT1200S (Leica Microsystems, Wetzlar, Germany) and stained with 0.1% (w/v) toluidine blue O in distilled water (DW). The sections were visualized by microscopy (DMRB, Leica Microsystems, Wetzlar, Germany).

Hasegawa K., Ichikawa A., Takeuchi H., Nakamura A, & Iwai H. (2023). Maintenance of Methyl-Esterified Pectin Level in Pollen Mother-Cell Stages Is Required for Microspore Development. Plants, 12(8), 1717.

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Hippocampal Slice Preparation from Mice

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Hemi-brain slices from the dorsal hippocampus were obtained from male KO and WT mice at 8 months of age. Animals were sacrificed by decapitation, and the brain was rapidly extracted and transferred into a slicing chamber containing ice-cold ACSF ([in mM] 124 NaCl, 26.2 NaHCO₃, 4.4 KCl, 1.3 MgSO₄, 1 NaH₂PO₄, 2.5 CaCl₂, 10 glucose, and 19.7 mannitol; pH 7.3–7.4) saturated with carbogen (95% O₂/5% CO₂). The brain was split into two hemispheres using a scalpel and sectioned into 400μm transverse slices using a vibratome (Leica VT1200s; Leica Microsystems, Concord, ON). Slices were then transferred into a submerged incubation chamber filled with ACSF, continuously bubbled with carbogen, maintained at 33.5°C, and allowed to recover for 2 h prior to recording.

Ghoweri A.O., Gagolewicz P., Frazier H.N., Gant J.C., Andrew R.D., Bennett B.M, & Thibault O. (2020). Neuronal Calcium Imaging, Excitability, and Plasticity Changes in the Aldh2–/– Mouse Model of Sporadic Alzheimer’s Disease. Journal of Alzheimer's Disease, 77(4), 1623-1637.

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Acute brain slice preparation

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A P7–P14 mouse was anesthetized in a sealed chamber containing the inhalation anesthetic isoflurane in an upward flowing BSL2 cabinet. After decapitation, the brain was quickly removed and immersed in the ice-cold oxygenated slicing solution. A coronal cut was made in the rear of the cortical region to separate motor cortex and cerebellum. A sagittal cut was made through the middle line of the vermis of the cerebellum. Both cortex and cerebellum were glued on the same cutting stage, 300–400 μM slices were cut with a LEICA VT1200S tissue slicer (Leica Microsystems, Wetzlar, Germany). From each brain 4 coronal cortical slices (400 μM) including motor cortex or/and 4 sagittal cerebellum slices (300 μM, lateral) were collected and moved into the incubation chamber with a transfer pipet. Slices were kept at 34°C for 30 min, then left to recover at room temperature for at least 60 min before electrophysiological recordings.

Tharaneetharan A., Cole M., Norman B., Romero N.C., Wooltorton J.R., Harrington M.A, & Sun J. (2020). Functional abnormalities of cerebellum and motor cortex in spinal muscular atrophy mice. Neuroscience, 452, 78-97.

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