Easysep human naive b cell isolation kit

Manufactured by STEMCELL

The EasySep™ Human Naive B Cell Isolation Kit is a magnetic cell separation system designed to isolate naive B cells from human peripheral blood mononuclear cells. The kit utilizes a cocktail of antibodies to label unwanted cells, which are then magnetically removed, leaving the desired naive B cell population.

Automatically generated - may contain errors

Lab products found in correlation

Interleukin 4 (il 4), by Immunotools (1 mentions)

Close spelling variants of this product

EasySep Human B Cell Isolation Kit EasySep B cell isolation kit EasySep cell isolation kit EasySep isolation kits Cell isolation kits EasySep kits Isolation kits

3 protocols using easysep human naive b cell isolation kit

Isolation of Naive B Cells from LN Patients

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples of patients with LN or healthy controls (HC) by density gradient centrifugation. CD19⁺ B cells were purified from PBMCs by positive selection using the EasySep™ Human CD19 Positive Selection Kit. Naive B cells were isolated using EasySep™ Human Naive B Cell Isolation Kit (STEMCELL Technologies). Cell purities were checked by FACS (>95%).

Li X., Zeng Q., Wang S., Li M., Chen X., Huang Y., Chen B., Zhou M., Lai Y., Guo C., Zhao S., Zhang H, & Yang N. (2021). CRAC Channel Controls the Differentiation of Pathogenic B Cells in Lupus Nephritis. Frontiers in Immunology, 12, 779560.

+ Open protocol

+ Expand

Naive B Cell Isolation and Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs were purified from blood by density gradient centrifugation. Naive CD27⁻ B cells were isolated with the EasySep Human Naive B cell Isolation Kit (STEMCELL Technologies) following the manufacturer’s instructions. Isolated naive B cells were plated in supplemented IMDM (Thermo Fisher Scientific) as described previously (Warnatz et al., 2009 (link)) at a concentration of 0.15 × 10⁶ cells/ml. Cells were stimulated with trimeric CD40L and IL-21 as described (Warnatz et al., 2009 (link)) in the presence or absence of IL-4 (25 ng/ml; Immunotools).

Kögl T., Chang H.F., Staniek J., Chiang S.C., Thoulass G., Lao J., Weißert K., Dettmer-Monaco V., Geiger K., Manna P.T., Beziat V., Momenilandi M., Tu S.M., Keppler S.J., Pattu V., Wolf P., Kupferschmid L., Tholen S., Covill L.E., Ebert K., Straub T., Groß M., Gather R., Engel H., Salzer U., Schell C., Maier S., Lehmberg K., Cornu T.I., Pircher H., Shahrooei M., Parvaneh N., Elling R., Rizzi M., Bryceson Y.T., Ehl S., Aichele P, & Ammann S. (2024). Patients and mice with deficiency in the SNARE protein SYNTAXIN-11 have a secondary B cell defect. The Journal of Experimental Medicine, 221(7), e20221122.

+ Open protocol

+ Expand

Isolation of Primary Naive B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary naive B cells were obtained from fresh buffy coats provided by the Institute for Transfusion Medicine and Gene Therapy, ITG Freiburg. Peripheral blood mononuclear cells were separated by Ficoll gradient centrifugation of whole blood and negatively selected using EasySep Human Naive B Cell Isolation kit (StemCell). Prior to the experiments, primary naive B cells were controlled for purity and rested overnight. The study was approved by the Institutional Review Board of the University of Freiburg (ethical vote nos. 507/16 and 336/16). Additional details can be found in SI Appendix, Methods.

Kläsener K., Jellusova J., Andrieux G., Salzer U., Böhler C., Steiner S.N., Albinus J.B., Cavallari M., Süß B., Voll R.E., Boerries M., Wollscheid B, & Reth M. (2021). CD20 as a gatekeeper of the resting state of human B cells. Proceedings of the National Academy of Sciences of the United States of America, 118(7), e2021342118.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!