Lipopolysaccharides (LPS) (purity ≥ 99%) were purchased from Sigma, Dulbecco’s Modified Eagle medium (DMEM) was purchased from Cytiva, and penicillin-streptomycin antibiotics (1000 IU/mL penicillin and 1000 μg/mL streptomycin) were purchased from Gibco, and fetal bovine serum (FBS) was purchased from HyClone. Cell Counting Kit 8 (CCK-8) was purchased from Sigma. RNA extraction kit, cDNA reverse transcription kit, and SYBR qPCR reagent were purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). The sequences of all primers were purchased from Shanghai Sangong (Shanghai, China). All other reagents were analytically pure.
Sybr qpcr reagent
Manufactured by Vazyme
Sourced in China
SYBR qPCR reagent is a real-time PCR reagent designed for quantitative gene expression analysis. It contains a SYBR Green I dye that binds to double-stranded DNA, allowing for the detection and quantification of target DNA sequences during the amplification process.
Automatically generated - may contain errors
Lab products found in correlation
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Cytiva (1 mentions)
Cdna reverse transcription kit, by Vazyme (1 mentions)
Rna extraction kit, by Vazyme (1 mentions)
Penicillin streptomycin antibiotic, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Merck Group (1 mentions)
Rt reagent kit, by Takara Bio (1 mentions)
Rna easy isolation reagent, by Vazyme (1 mentions)
2 protocols using sybr qpcr reagent
1
LPS-Induced Cell Response Analysis
2
Quantitative Gene Expression Analysis
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Total RNA was extracted with RNA-easy Isolation Reagent (#R701, Vazyme). After quantification, the same amount of RNA was reverse transcribed with an RT Reagent Kit (RR037A, Takara). Quantitative PCR was performed using SYBR qPCR reagent (Q111, Vazyme). GAPDH for cells and 18S rRNA for tissues were used as reference genes. The following primers were used: SLC2A3-F: GTCGGACTCTTCGTCAACCG, SLC2A3-R: AGAGGCCAATAACCAAGCGA; CXCR4-F: CTCCTCTTTGTCATCACGCTTCC, CXCR4-R: GGATGAGGACACTGCTGTAGAG; CCR1-F: GCATCATCATTTGGGCCCTG, CCR1-R: AAAGTGAAGGCTGCAGGTGT; CCR8-F: TGGCTGTTGTCCATGCCGTGTA, CCR8-R: TGGGATGGTAGCCATAATGGCG; CCL2-F: GCAATCAATGCCCCAGTCAC, CCL2-R: CTTTGGGACACTTGCTGCTG; CCL13-F:CAGCAGGTGTCCCCAGAAG, CCL13-R: TTCAGGGTGTGAGCTTTCCG; 18 s rRNA-F: CTGGATACCGCAGCTAGGAA, 18 s rRNA-R: GAATTTCACCTCTAGCGGCG; GAPDH-F: TCGACAGTCAGCCGCATCTT, GAPDH-R: GAGTTAAAAGCAGCCCTGGTG.
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