The surface morphology of the electrospun membranes was observed using a scanning electron microscopy analysis in a scanning electron microscope EVOMA25 (Carl Zeiss, Oberkochen, Germany) instrument with acceleration voltage of 5–10 kV. Samples were carefully sectioned with an approximate size of 8 mm length by 0.5 mm, and then dried in a desiccator and sputtered with carbon for 60 s in an evaporator, Quorum Q150R E. Energy dispersive X-ray (EDX) attachment of the EVOMA25 SEM analysis was carried out to confirm the presence of the particles. Fiber diameter was performed using an image visualization software ImageJ-Fiji J with the plugin DiameterJ developed by Upper Austria University of Applied Sciences.
Evo ma 25
Manufactured by Zeiss
Sourced in Germany
The EVO MA 25 is a scanning electron microscope (SEM) designed for materials analysis. It provides high-resolution imaging and analytical capabilities to investigate the surface and internal structure of a wide range of materials. The EVO MA 25 is equipped with advanced features that enable users to obtain detailed information about the composition, morphology, and properties of their samples.
Automatically generated - may contain errors
Lab products found in correlation
E 1010 ion sputtering device, by Hitachi (2 mentions)
Am400, by Renishaw (1 mentions)
Q150r es device, by Quorum Technologies (1 mentions)
Xflash 6, by Bruker (1 mentions)
Evo25, by Zeiss (1 mentions)
24 well plate, by Avantor (1 mentions)
Ckx41, by Olympus (1 mentions)
Penicillin, by Biowest (1 mentions)
Axio csm 700, by Zeiss (1 mentions)
Jem 2100 analytical electron microscope, by JEOL (1 mentions)
35 protocols using evo ma 25
1
Scanning Electron Microscopy of Electrospun Membranes
2
Characterization of PLA and PLLA Microspheres
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Microspheres of PLA and PLLA were characterized using scanning electron microscopy (SEM) using Zeiss EVO MA25 (Zeiss, Oberkochen, Germany) with the back -scattered electron detector and accelerating voltage of 20 kV. The captured SEM using Zeiss EVO MA25 images allowed us to determine the shape and size of the investigated powders.
3
Vascular Graft Membrane Morphology Analysis
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A scanning electron microscope (SEM) EVOMA25 (Carl Zeiss, Oberkochen, Germany) was used to characterize the vascular graft membrane morphology. The coating procedure was conducted using gold. Images were acquired using conventional SEM operating at an accelerated voltage of 5 kV with a working distance of 9 mm. The images were analyzed using ImageJ software (version 1.44p, U.S. National Institutes of Health, Bethesda, MD, USA) to determine the diameter of fibers and alignment. Fiber diameter measurements were conducted at 200 random positions. Distribution and average diameter were computed and reported. The alignment analysis was achieved using ImageJ software.
4
Metallographic Analysis of Aluminum Alloy
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Metallographic analyses were performed with an optical microscope after Barker etching. The solution for etching is composed of 1.8% fluoroboric acid in water and applying a tension in a continuous current of 20–45 V dc, for up to 2 min.
All samples were tested in the T4 condition (solubilization, tempering, and natural aging).
The grain size has been determined according to ASTM (American Society for Testing and Materials International, West Conshohocken, PA, USA) size. Reported values correspond to the number of grains per square inch at 100X magnification.
The fracture surfaces were observed by means of a scanning electron microscope (SEM) (Zeiss Evo MA 25). Moreover, a microanalysis using energy-dispersive X-ray spectroscopy (Oxford AZtec Energy X Max 80) was carried out on the fracture surface to detect the elements’ distribution.
All samples were tested in the T4 condition (solubilization, tempering, and natural aging).
The grain size has been determined according to ASTM (American Society for Testing and Materials International, West Conshohocken, PA, USA) size. Reported values correspond to the number of grains per square inch at 100X magnification.
The fracture surfaces were observed by means of a scanning electron microscope (SEM) (Zeiss Evo MA 25). Moreover, a microanalysis using energy-dispersive X-ray spectroscopy (Oxford AZtec Energy X Max 80) was carried out on the fracture surface to detect the elements’ distribution.
5
Metallic Powder Characterization and LPBF Fabrication
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The AISI 316L metallic powder was obtained from Renishaw (Renishaw, Wotton-under-Edge, England). Using scanning electron microscopy (SEM) EVOMA25 (Carl Zeiss, Oberkochen, Germany), the average measured particle size (n = 55) was 28.93 ± 7.70 μm (D10: 20.13 μm, D90: 37.56 μm). The chemical composition, provided by the supplier, is presented in Table 1 .
The LPBF fabrication was conducted in a Renishaw AM400 machine equipped with a ytterbium fiber laser with 400 W of maximum power. The processing parameters, based on a previously published work [30 (link)], are shown inTable 2 . The cylindrical samples were printed vertically, with the z-axis in Figure 1 and Figure 2 normal to the printing bed. Then, they were removed from the building platform by a wire electrical discharge machine (EDM). Finally, the samples were cleaned with bidistilled water in an ultrasonic bath for 30 min to remove the unmelted and loose powder.
The LPBF fabrication was conducted in a Renishaw AM400 machine equipped with a ytterbium fiber laser with 400 W of maximum power. The processing parameters, based on a previously published work [30 (link)], are shown in
6
Analyzing Al-based MWCNTs Nanocomposite Morphology
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To investigate the cross-sectional surface morphology of the Al-based MWCNTs nanocomposites, SEM (ZEISS, EVO MA 25, Oberkochen, Germany) microscope experimental measurements were performed. The system was operated at 20 kV as accelerating voltage considering 7.5 mm of work distance. The corresponding analyses were performed by taking secondary electrons (SE) and backscattered electrons (BSE) images to investigate the morphology and the chemical composition, respectively. The MWCNTs distribution was investigated by analyzing energy dispersive spectroscopy (EDS) elemental mapping images.
7
Scanning Electron Microscopy of Materials
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The materials were stuck onto carbon rings and covered with gold and palladium (60:40; sputter current—40 mA; sputter time—50 s) using a Quorum sputter coater (Quorum Technologies Ltd., Laughton, UK) and examined under a Zeiss EVO MA25 scanning electron microscope (Carl Zeiss, Jena, Germany).
8
Characterizing PTFE Nanofiber Membranes
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The morphologies of PTFE nanofiber membranes were investigated with a FESEM equipped with an X-ray energy dispersive spectrometer (EDS) (EVO MA 25, ZEISS, Germany). The PTFE nanofiber membranes were frozen in liquid nitrogen, fractured to obtain fragments, and sputtered with platinum using a HITACHI E-1010 Ion Sputtering device for FESEM observation. EDS detector was used to determine the existence of PVA.
9
Characterization of Surface Topography and Wettability
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Scanning electron microscopy (SEM, Zeiss-EVO MA25, Germany) was used to capture the microscopic topography images of the sample surface, and the surface was sprayed with gold and imaged in a low vacuum mode to ensure image quality. Three-dimensional morphology of sample surface was obtained by laser scanning confocal microscope (LSCM, Zeiss-LSM700, Germany). The chemical composition of the sample surface was analyzed by energy dispersive spectrometer (EDS, Oxford-X-Max 20, The United Kingdom) in a plane scanning mode with an area of 50 μm × 50 μm, and the results were average values of 5 different regions. The wettability of the sample surface was measured by developed contact angle measuring instrument using ellipse method (measurement range: 0–180°, reading resolution: 0.01°). The stopwatch was used to record the time and the image was taken by a CCD camera. In order to solve the problem of wettability deviation caused by the uniformity of the surface obtained by the WEDM, the contact angle (CA) was measured repeatedly at five different positions on the surface of the sample.
10
Characterization of CIP Powder Morphology
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The morphology and particle distribution were observed by scanning electron microscopy (SEM, Evo MA 25, Carl Zeiss, Oberkochen, Germany). For the morphology, a small amount of CIP powder was deposited on carbon tape and observed using an accelerating voltage of 20.00 kV and a working distance of 9.0 mm. To obtain the particle size distribution, around 200 particles recorded by SEM were used to estimate the particle mean size distribution by Digimizer 4.6.1 software (MedCale Software Ltd, Ostend, Belgium). With respect to the particle distribution in the elastomer, a cross section of the samples was made and the surface was washed with 50% isopropanol. Subsequently, the samples were observed by SEM using an accelerating voltage of 10 KV and a working distance of 12 mm.
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