Following inoculation on 96‐well plates with DMEM free of serum, cells were incubated for required time, and each well was added with 10‐μl Cell Counting Kit‐8 (CCK‐8) reagents (Beyotime, Nantong, China). At 1 hr after incubation, TECAN infinite M200 multimode microplate reader (Tecan, Mechelen, Belgium) was applied to evaluate A450, and 5‐Ethynyl‐2'‐deoxyuridine (EdU) assay was carried out for evaluation of cell proliferation capacity. The same experiment was repeated 3 times at least.
Cell counting kit 8 cck 8 reagent
Manufactured by Beyotime
Sourced in China, United States
The Cell Counting Kit-8 (CCK-8) reagent is a colorimetric assay used for the determination of cell viability and cytotoxicity. It employs a water-soluble tetrazolium salt that is reduced by dehydrogenases in viable cells to produce a colored formazan dye, which can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (8 mentions)
Microplate reader, by Bio-Rad (5 mentions)
Microplate reader, by Agilent Technologies (4 mentions)
Microplate reader, by Tecan (2 mentions)
Crystal violet, by Beyotime (2 mentions)
Elisa microplate reader, by Thermo Fisher Scientific (1 mentions)
Cck 8 reagent, by Agilent Technologies (1 mentions)
Capecitabine, by Sinopharm (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Glacial acetic acid, by Sinopharm (1 mentions)
Mda mb 231 human breast cancer, by American Type Culture Collection (1 mentions)
Multidetection microplate reader, by Bio-Rad (1 mentions)
Novocyte flow cytometer, by Agilent Technologies (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Total rna extraction kit, by Solarbio (1 mentions)
Ldh cytotoxicity assay kit, by Beyotime (1 mentions)
Microplate spectrophotometer, by Thermo Fisher Scientific (1 mentions)
63 protocols using cell counting kit 8 cck 8 reagent
1
Cell Proliferation Assay with CCK-8 and EdU
2
Quantifying HUVEC Proliferation Using CCK-8
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HUVECs were seed into 96-well plates (5×103 cell/well) for 24 hours. Subsequently, Cell Counting Kit-8 (CCK-8) reagents (10 μL/well) (Beyotime, Shanghai, China) were added into each well for about 4 hours. Finally, the absorbance was determined by a microplate reader at 450 nm in the indicated time.
3
Cell Viability Assay via CCK-8
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Briefly, 1 × 103 preprocessed SW1990 cells were seeded in a 96-well plate. The medium was replaced with 100 µl fresh medium with 10% Cell Counting Kit-8 (CCK-8) reagents (Beyotime Biotech, China) in each well at 0, 24, 48, and 72 h and incubated at 37 °C for additional two hours. Finally, the absorbance at 450 nm was measured using an ELISA microplate reader (Thermo, USA).
4
HUVEC Cell Viability Assessment
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At specified time points (0, 24 and 48 h), HUVECs were seed into 96-well plates (5 × 103 cell/well) and treated wtih Cell Counting Kit-8 (CCK-8) reagents (10 μl/well) (Beyotime, Shanghai, China). Following a 2-h incubation at 37 °C, the absorbance was measured at 450 nm using a microplate reader (BioTek, Winooski, VT, USA).
5
Oxidized LDL Impacts Endothelial Cell Viability
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A 300ul ECs suspension (4 x 10 4 cells/ well) was incubated with different concentrations of ox-LDL for 24 hours and 48 hours. Then a cell Counting Kit-8 (CCK-8) reagents (30 μL/well) (Beyotime, Shanghai, China) were added into each well for 2 hours. The absorbance was measured at 450 nm with a microplate reader.
6
Cell Viability Assessment with CCK8
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The transfected cells were collected and inoculated on 96-well plates at 2000 cells per well. After addition of 10 μL Cell Counting Kit-8 (CCK8) reagent (Shanghai Beyotime Biotechnology Co.) into each well, detection of the absorbance (OD) value at 450 nm wavelength was performed with a microplate reader at different times.
7
Cell Proliferation Assay using CCK-8
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Cells (3 × 10 3 cells/well) were seeded into 96-well plates, and 100 μL of DMEM medium supplemented with 10% FBS was added. Cells were incubated for 0, 24, 48, and 72 h. Each well was then supplemented with Cell Counting Kit-8 (CCK-8) reagent (10 μL; Beyotime Biotechnology), and the plates were incubated at 37°C for 1 h. Optical density at 450 nm (OD 450 ) was measured using a microplate reader (model 1681130; Bio-Rad, Hercules, USA).
8
Cell Viability Assay by CCK-8
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Cells were seeded in 96-well plates at a density of 5000 cells per well in 100 μL of complete medium. At the indicated time points, 10 μL of the Cell Counting Kit-8 (CCK-8) reagent (Beyotime, China) was added to each well, and the plate was incubated at 37 °C for an additional 1 h. The absorbance in each well was measured at 450 nm using a microplate reader.
9
Cell Viability Assay Using CCK-8
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Cell growth was determined using the cell counting kit-8 (CCK-8) reagent (Beyotime Biotechnology). Adjust the cell concentration to 1 × 104, add 100 ul of mixed cell suspension to 96-well plates. As instructed in the manual, stimulated with the indicated chemicals and then incubated with the CCK-8 reagent. Absorbance was measured at 490/630 nm on a microplate reader, the time interval is six hours.
10
Cell Viability Assay of C6 Cells
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The C6 cells from the LV-NC and LV-BLBP groups were seeded at a density of 2×103 cells/well into 96-well plates. All cells were cultured in 100 µl of 10% and 1% FBS culture medium for 24, 48, 72, 96 and 120 h. For the cell viability assay, the Cell Counting Kit-8 (CCK-8) reagent (Beyotime, Shanghai, China) was used. Briefly, the 10% or 1% FBS culture medium was discarded, after which 10 µl of CCK-8 reagent and 90 µl of serum-free DMeM/F12 medium were added to each well and incubated for 1 h. Optical density (OD) was measured using the Synergy 2 enzyme mark instrument (BioTek, Winooski, VT, USA) at a wavelength of 450 nm.
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