(−)-Nicotine hydrogen tartrate [(−)-1-methyl-2-(3- pyridyl) pyrrolidine (+)-bitartrate] was purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). PNU120596 [1-(5-chloro-2, 4-dimethoxy-phenyl)-3-(5-methyl-isoxazol-3-yl)] and PNU282987 [N-(3R)-1 Azabicyclo [2.2.2] oct-3-yl-4-chlorobenzamide] were obtained from the National Institute on Drug Abuse (NIDA) supply program (Bethesda, MD). NS6740 (1, 4-diazabicyclo [3.2.2] nonan-4-yl (5-(3-(trifluoromethyl) phenyl) furan-2-yl) methanone). NS1738 was purchased from Tocris Biosciences (Minneapolis, MN). Morphine sulfate was obtained from the National Institute on Drug Abuse (Baltimore, MD, USA). Nicotine, morphine, NS6740, and PNU282987 were dissolved in physiological saline. NS1738 and PNU120596 were dissolved in a mixture of 1:1:18 [1 volume ethanol/1 volume Emulphor-620 (Rhone-Poulenc, Inc., Princeton, NJ) and 18 volumes distilled water]. Nicotine, morphine, and PNU282987 were injected subcutaneously (s.c.), while all other drugs were administered intraperitoneally (i.p.). The nicotine solution pH was neutralized to pH 7.0 with sodium bicarbonate as needed. Freshly prepared solutions were given to mice at 10 ml/kg, s.c. Doses are expressed as the free base of the drug.
Ns 1738
Manufactured by Bio-Techne
Sourced in United Kingdom
NS-1738 is a laboratory product manufactured by Bio-Techne. It is a piece of equipment designed for use in scientific research and analysis. The core function of NS-1738 is to perform specific tasks within a laboratory setting, but no further details about its intended use can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using ns 1738
1
Pharmacological Evaluation of Nicotinic Ligands
2
Synthesis and Characterization of Compound B
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(R)-N-(1-azabicyclo[2.2.2]oct-3-yl)(5-(2-pyridyl)thiophene-2-carboxamide) (compound B) was synthesised by Lilly Research Laboratories according to methods described previously [28 ]. 4-(1-napthyl)-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-8-sulfonamide (TQS) and 4-(4-bromophenyl)-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-8-sulfonamide (4BP-TQS) were obtained from Chembridge Corporation (San Diego, CA). PNU-120596, NS-1738 and 5-Iodo-A-85830 were obtained from Tocris Biosciences (Bristol, UK). 4-(5-(4-Chlorophenyl)-2-methyl-3-proprionyl-1H-pyrrol-1-yl)-benzenesulfonamide (A-867744) was provided by Abbott laboratories. Human neurons (iCell-Neurons) derived from induced pluripotent stem cells were obtained from Cellular Dynamics International (Madison, WI, USA). As outlined in the product specifications and the corresponding user guide, the iCell-Neurons were generated by using the proprietary differentiation and purification protocols provided by the supplier (see product userguide at and http://www.cellulardynamics.com/products/lit/CDI_iCellNeuronsUsersGuide140826.pdf ).
According to the information provided by the supplier (CDI) they represent a mixture of post-mitotic neural subtypes comprising primarily of GABAergic and glutamatergic neurons.
According to the information provided by the supplier (CDI) they represent a mixture of post-mitotic neural subtypes comprising primarily of GABAergic and glutamatergic neurons.
3
Triazole Synthesis and Quinoline Compounds
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As is described in more detail in the Supporting information , the following compounds were prepared by a modification of a literature procedure for triazole synthesis (El Kaim et al., 2010 ): 4-(3-(4-bromophenyl)-5-phenyl-1H-1,2,4-triazol-1-yl)benzenesulfonamide (TBS-345), 4-(3-(4-bromophenyl)-5-(4-methoxyphenyl)-1H-1,2,4-triazol-1-yl)benzenesulfonamide (TBS-346), 4-(5-benzyl-3-(4-bromophenyl)-1H-1,2,4-triazol-1-yl)benzenesulfonamide (TBS-516), 4-(3-(4-bromophenyl)-5-propyl-1H-1,2,4-triazol-1-yl)benzenesulfonamide (TBS-546) and 4-(3-(4-bromophenyl)-5-phenethyl-1H-1,2,4-triazol-1-yl)benzenesulfonamide (TBS-556). Synthesis of cis-cis-4-(4-bromophenyl)-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-8-sulfonamide (4BP-TQS) and cis-cis-4-(napthalen-1-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-8-sulfonamide (TQS) has been described previously (Gill et al., 2012 (link)). NS-1738 was obtained from Tocris Bioscience.
4
Nicotinic Receptor Modulation in Mice
5
Synthesis and Characterization of Pharmacological Probes
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5-Hydroxyindole (5-HI), acetylcholine (ACh), and probenecid were purchased from Sigma-Aldrich (St Louis, MO, USA). NS-1738 (N-(5-Chloro-2-hydroxyphenyl)-N'-[2-chloro-5-(trifluoromethyl)phenyl]urea), PNU-120596 (N-(5-Chloro-2,4-dimethoxyphenyl) -N'-(5-methyl-3isoxazolyl)-urea), (±)-epibatidine hydrochloride were obtained from Tocris Biosciences (Bristol, UK). Fluo-4 was purchased from Molecular Probes (Eugene, OR, USA). PAM-2 (3-furan-2-yl-Np-tolylacrylamide), -3 (3-furan-2-yl-N-o-tolylacrylamide) and -4 (3-furan-2-yl-Nphenylacrylamide) were synthesized as in Arias et al. (2011) .
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