Ve cadherin

Manufactured by R&D Systems

Sourced in United States, United Kingdom

VE-Cadherin is a cell adhesion molecule that plays a critical role in the formation and maintenance of vascular endothelial cell-cell junctions. It is a transmembrane protein that mediates homotypic cell-cell adhesion, contributing to the regulation of vascular permeability and integrity.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 510 meta, by Zeiss (4 mentions) Triton x 100, by Merck Group (3 mentions) Axio observer z1, by Zeiss (3 mentions) Pecam 1, by R&D Systems (2 mentions) Gibco opti mem glutamax, by Thermo Fisher Scientific (2 mentions) Desmin, by Abcam (2 mentions) Cleaved notch1, by Cell Signaling Technology (2 mentions) Jagged1, by R&D Systems (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Eclipse 80i microscope, by Nikon (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Lyve 1, by Abcam (2 mentions) Fluoromount aqueous mounting medium, by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Alexa fluor 647, by Abcam (1 mentions) Csf1r, by Abcam (1 mentions) Runx1, by Abcam (1 mentions) Ter119 pe, by BD (1 mentions) C kit fitc, by Thermo Fisher Scientific (1 mentions) Alexa fluor tagged secondary antibodies, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-VE-cadherin (9 citations) Goat anti-VE-cadherin (5 citations) Mouse VE-Cadherin (4 citations) VE-Cadherin antibody (2 citations) Anti-Human VE-Cadherin Monoclonal Antibody (2 citations) Goat anti-mouse VE-cadherin (2 citations) Goat anti VE-cadherin antibody (2 citations)

33 protocols using ve cadherin

Multiparametric Flow Cytometry and IHC Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used for flow cytometry and immunohistochemistry analysis: CD31 (BD Bioscience, #553371; DSHB, 2H8), CD34-FITC (eBiosciences, #11-0341), Ter119-PE (BD Biosciences, #553673), Runx1 (Abcam, ab92336), c-kit-FITC (eBiosciences, #11-1171), CSF1R (Abcam, #Ab32633; eBioscience AFS98), and VE-Cadherin (R&D, #AF1002). Alexa-Fluor tagged secondary antibodies (Invitrogen) were used for all non-conjugated primary antibodies with the exception of anti-Armenian Hamster secondary (AlexaFluor647, Abcam).

Kasaai B., Caolo V., Peacock H.M., Lehoux S., Gomez-Perdiguero E., Luttun A, & Jones E.A. (2017). Erythro-myeloid progenitors can differentiate from endothelial cells and modulate embryonic vascular remodeling. Scientific Reports, 7, 43817.

+ Open protocol

+ Expand

Aortic Ring Sprouting Assay for Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The aortic ring sprouting assay was performed as previously (20 (link)). Briefly, aortas were harvested from 8 to 10 weeks old mice and washed with DMEM/F14 medium (Gibco, Carlsbad, USA) supplemented with 100 U/mL penicillin, and 100 μg/mL streptomycin. The dissected aortas were subsequently cleaned, sectioned in 12–16 rings of 1 mm length, and embedded in collagen type 1 (Corning, New York, USA). After polymerization of the collagen gel, microvascular endothelial cell growth medium (PeloBiotech, Planegg, Germany) supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin, and 2% murine serum (BD) was added into the well. Tube-like structures were allowed to develop over 7 days. Thereafter, samples were fixed in 4% PFA and endothelial cells were visualized using antibodies against CD31 (Dianova, Hamburg, Germany) and VE-Cadherin (R&D), while NG2 (Merck, Darmstadt, USA) staining was employed to detect pericytes. The total volume of vascular and perivascular sprouting in each explant was calculated trough the IMARIS-BITPLANE 9.3 software. Additionally, total sprout length was measured with ImageJ.

Fink A.F., Ciliberti G., Popp R., Sirait-Fischer E., Frank A.C., Fleming I., Sekar D., Weigert A, & Brüne B. (2019). IL27Rα Deficiency Alters Endothelial Cell Function and Subverts Tumor Angiogenesis in Mammary Carcinoma. Frontiers in Oncology, 9, 1022.

+ Open protocol

+ Expand

Immunostaining of Cell Cultures on Scaffolds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells on the scaffolds were fixed on day 7 with 4% paraformaldehyde (PFA) in PBS (Affymetrix, Cleveland, OH, USA) for 15 min and permeabilized with 0.05% Triton^®X-100 (in PBS, Sigma-Aldrich, Taufkirchen, Germany) for 10 min. For MSCs mono-cultures, cells were stained with Phalloidin-TRITC (1:100 in PBS, Sigma-Aldrich) for 30 min and then Höchst (2 µg/mL in PBS, Sigma-Aldrich) for 10 min. The co-cultures were incubated with VE-Cadherin (R&D, Minneapolis, MN, USA) antibodies (1:50 diluted in PBS with 1% bovine serum albumin (BSA)) for 2 h. After being washed three times with PBS, the cells were incubated with the secondary antibody (1:1000 in 1% BSA) and Phalloidin-TRITC (1:100) for 45 min, followed by 10 min incubation with Höchst (2 µg/mL in PBS). The immuno-stained samples were kept in PBS for visualization with confocal laser scanning microscope (CLSM, LSM 800, Zeiss, Germany) affiliated with ZEN image system.

Wang F., Saure L.M., Schütt F., Lorich F., Rasch F., Nia A.S., Feng X., Seekamp A., Klüter T., Naujokat H., Adelung R, & Fuchs S. (2022). Graphene Oxide Framework Structures and Coatings: Impact on Cell Adhesion and Pre-Vascularization Processes for Bone Grafts. International Journal of Molecular Sciences, 23(6), 3379.

+ Open protocol

+ Expand

Immunofluorescence Antibody Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used for cell or embryo immunofluorescence were used at a dilution of 1/100 unless otherwise specified. Antibodies were raised against Isl1 (Developmental Hybridoma bank, Iowa University 1/50), Tbx20 (novus biological H00057057-B01), Msx1 (Abcam ab93287), Tbx3 (Santa Cruz sc-178721), aggrecan (Chemicon AB1031), versican (Chemicon AB1033), smooth muscle actin (Sigma A-2547), Filamin A (Epitomics, CA, USA), vimentin dylight 550 (Novus biological, VM452, 1/200), periostin (Abcam ab140141), CD31 (BD pharmigen, WM59), VE-cadherin (R&D systems/MAB9381), Sox9 (a gift from Pr Wegner, University of Nurnberg, and Santa-Cruz Sc17431), GATA5 (Abcam ab11877), Hyaluronan-binding protein (Millipore 385911), collagen I (Abcam 34710), anti-Patched1 (Merck-Millipore, France, 06–1102), human Lamin A/C (Novacastra, NCL-LAM-A/C), and anti-NFATc (Santa-Cruz 17844 H10).

Neri T., Hiriart E., van Vliet P.P., Faure E., Norris R.A., Farhat B., Jagla B., Lefrancois J., Sugi Y., Moore-Morris T., Zaffran S., Faustino R.S., Zambon A.C., Desvignes J.P., Salgado D., Levine R.A., de la Pompa J.L., Terzic A., Evans S.M., Markwald R, & Pucéat M. (2019). Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis. Nature Communications, 10, 1929.

+ Open protocol

+ Expand

Endothelial Cell Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

We observed CAR and endothelial cell-to-cell junctional protein expression level; protein was extracted from both WT and KO endothelial cells. Immunoblot analysis used an ECL detection system as described previously [24 (link)]. Primary antibodies were used; anti-CAR (Santa Cruz Biotechnologies, Dallas, TX, USA), PECAM-1, VE-cadherin (R&D system, Minneapolis, MN, USA), FLK-1 (Sigma-Aldrich, Inc. St. Louis, MO, USA), integrin-β1D (Zymed Laboratories, Inc. South San Francisco, CA, USA), and GAPDH (Santa Cruz Biotechnologies, Dallas, TX, USA). Protein expression levels were evaluated with Image Lab software (Bio-Rad laboratories, Hercules, CA, USA) based on the signal intensity of each protein using GAPDH as an internal control for protein loading.

Park J.H., Shin H.H., Rhyu H.S., Kim S.H., Jeon E.S, & Lim B.K. (2021). Vascular Endothelial Integrity Affects the Severity of Enterovirus-Mediated Cardiomyopathy. International Journal of Molecular Sciences, 22(6), 3053.

+ Open protocol

+ Expand

Immunostaining of Cultured Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cultured endothelial cells were fixed with ice-cold butanol for 15 min at room temperature, followed by blocking and permeabilization with 2% BSA and 1% Triton X-100 in PBS and incubated with primary antibodies against CAR (1:100; Santa Cruz, Dallas, TX, USA), VE-cadherin, and PECAM-1 (1:500; R&D system, Minneapolis, MN, USA). The target proteins were visualized with the secondary antibodies conjugated with fluorescence (Alexa fluor 488 and 594, 1:400; Invitrogen, South San Francisco, CA, USA) and Hoechst nuclear stain. Fluorescence images were taken and processed using a fluorescent microscope (Olympus, Waltham, MA, USA) [23 (link)].

+ Open protocol

+ Expand

Western Blot Analysis of Endothelial Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analyses were performed on lysis of HLMVEC exposed to cytomix with or without treatment with MSCs or MSC MVs as previously described 16 using antibody for phosphorylated myosin light chain 2 (1:1,000 dilution, Cell Signaling), VE‐cadherin (1 μg/ml, R&D Systems), ZO‐1 (1:1,000, Cell Signaling) and β‐actin (R&D Systems) or glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (1:1,000, Cell Signaling) as loading controls. GTP‐bound Rac1/2/3 were measured in protein lysates of HLMVECs with a commercially available activation kit (G‐LISA, kit #BK125, Cytoskeleton, Inc., Denver, CO, http://www.cytoskeleton.com/) according to the manufacturer's instructions.

Hu S., Park J., Liu A., Lee J., Zhang X., Hao Q, & Lee J. (2018). Mesenchymal Stem Cell Microvesicles Restore Protein Permeability Across Primary Cultures of Injured Human Lung Microvascular Endothelial Cells. Stem Cells Translational Medicine, 7(8), 615-624.

+ Open protocol

+ Expand

Aortic Ring Assay for Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The aortic ring assay was performed as described previously [43 (link)]. Three ApoE3*Leiden mice, 4–8 weeks old, were anesthetized and the aorta was dissected. Each aorta was cut in 1 mm rings, and serum-starved in Gibco™ Opti-MEM™ GlutaMAX (51985034, ThermoFisherScientific) overnight at 37 °C and 5% CO₂. On the next day, each ring was mounted in a well of a 96-well plate in 70 µL of 1.0 mg/mL acid-solubilized collagen type-I (11179179001, Roche Diagnostics) in DMEM (12634010, ThermoFisher Scientific). After collagen polymerization, Gibco™ Opti-MEM™ GlutaMAX supplemented with 2.5% FCS and 30 ng/mL VEGF (recombinant mouse VEGF-164) was added with PC-mAb (10 µg/mL and 100 µg/mL). The rings were cultured for 7 days and photographed using Axiovert 40c microscope. The number of sprouts were counted manually. For immunohistochemistry, rings were formalin-fixed and permeabilized with 0.2% Triton X-100 (11332481001, Merck). Rings were stained with ACTA2, CD31 and VE-Cadherin (AF1002, R&D Systems). Extended focus pictures were made with the Pannoramic SCAN II and quantified with Image J.

Baganha F., Sluiter T.J., de Jong R.C., van Alst L.A., Peters H.A., Jukema J.W., Delibegovic M., Pettersson K., Quax P.H, & de Vries M.R. (2022). Phosphorylcholine Monoclonal Antibody Therapy Decreases Intraplaque Angiogenesis and Intraplaque Hemorrhage in Murine Vein Grafts. International Journal of Molecular Sciences, 23(21), 13662.

+ Open protocol

+ Expand

Whole-Mount Retinal Immunostaining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Retinas for whole-mount were fixed in 4% PFA for 2 hours at room temperature, or overnight at 4 °C. After fixation, retinas were blocked and permeabilized in blocking buffer (1% BSA and 0.5% Triton X-100 in PBS) overnight at 4°C. For mouse antibodies, samples were washed and blocked with mouse IgG blocking reagent as suggested by the manufacturer (MKB-2213 Vector, CA, USA) for 1 hour at room temperature. Primary antibodies were diluted in blocking buffer and incubated overnight at 4°C. The following antibodies were used: VE-cadherin (1:200, AF1002 R&D systems, Abingdon, UK), α-smooth muscle actin-cy3 (1:500, C6198 Sigma, Taufkirchen, Germany), N-cadherin (1:200, 33-3900 lifetechnologies, CA, USA), collagen IV (1:500, 1340-01 southern biotech, AL, USA), presenilin 1 (1:200, MAB1563 Millipore, Darmstadt, Germany) and desmin (1:500, ab15200 abcam, Cambridge, UK). For secondary detection Alexa Fluor-coupled secondary antibodies (1:200) were used. Cell nuclei were visualized with DAPI (0.2 ug/mL, D9542 Sigma). After antibody staining retinas were post-fixed with 4% PFA for 10 minutes before flat-mounting in mounting medium (Dako).

Hu J., Dziumbla S., Lin J., Bibli S.I., Zukunft S., de Mos J., Awwad K., Frömel T., Jungmann A., Devraj K., Cheng Z., Wang L., Fauser S., Eberhart C.G., Sodhi A., Hammock B.D., Liebner S., Müller O.J., Glaubitz C., Hammes H.P., Popp R, & Fleming I. (2017). Inhibition of soluble epoxide hydrolase prevents diabetic retinopathy. Nature, 552(7684), 248-252.

+ Open protocol

+ Expand

Quantification of Angiogenic Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies for PD-1, PD-L1 (B7-H1), Ly6G, ICAM-1, CD31, VE-Cadherin and MIP-2 were obtained from R&D Systems, Minneapolis, MN; anti-CD31 ELISA kit and mouse CBA cytokine assay kit (IL-6, TNF-α, MCP-1) from BD Bioscience, San Diego, CA; and Ang-1 and Ang-2 cytokine assay kits from Life Sciences Advanced Technologies, St. Petersburg, FL. Tie2 and phosphorylated Tie2 ELISA kits purchased from R&D Systems, Minneapolis, MN. All other chemicals were analytical reagent grade.

Lomas-Neira J., Monaghan S.F., Huang X., Fallon E.A., Chung C.S, & Ayala A. (2018). Novel Role for PD-1:PD-L1 as Mediator of Pulmonary Vascular Endothelial Cell Functions in Pathogenesis of Indirect ARDS in Mice. Frontiers in Immunology, 9, 3030.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!