Advia 1650

A complete blood count or hemogram was done in an automated analyzer (Cell-Dyn 3700,
Abbott Diagnostics, Chicago, Illinois, US); serum ALT, AST, CK, CRP and postprandial
lipid profile analyses were run on an automated chemistry analyzer ADVIA 1650 (Bayer
Diagnostics, Greenburgh, NY, US) and serum hs-CRP was measured by an immunometric
assay (Immulite 2000, DPC, Medlab) using the appropriate chemical reagents, controls
and protocols of the manufacturers. The TBARS assay was done according to Wasowicz et al. (1993) (link) and the
fluorescence was measured with a Jasco FP-777 spectrofluorometer (excitation: 525 nm,
emission: 547 nm).

Fasting venous blood samples were collected from individual healthy controls and MCD patients. One portion of blood was used for preparing peripheral blood mononuclear cells (PBMCs) by density-gradient centrifugation using Ficoll-Paque Plus (Amersham Biosciences, Little Chalfont, UK) and the remaining blood samples were centrifuged for preparing serum samples. The numbers of leukocytes and lymphocytes were examined and the concentrations of serum triglycerides, cholesterol, uric acid, and albumin were determined using ADVIA 1650 biochemical analyzer (Bayer, Pittsburg, PA, USA). In addition, 24 h urine samples were collected from individual participants, and the concentrations of urinary proteins and microscopic hematuria were measured. The values of estimated glomerular filtration rate (eGFR) of individual participants were calculated using the revised eGFR formula [17 (link)].

Height and body weight were measured to the nearest 0.1cm or 0.2kg, respectively. Blood pressure (BP) was measured in the sitting position after at least 5 minutes of rest. Blood samples were obtained after an overnight fast of at least 8 h, and biochemical assays including plasma glucose, total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) were measured using the ADVIA 1650 chemistry analyzer (Bayer HealthCare Ltd., Tarrytown, NY, USA). Hemoglobin A1c (HbA1c) level was measured using high-performance liquid chromatography (Variant II; BioRad Laboratories, Hercules, CA, USA)
The questionnaire included questions on sociodemographic information, lifestyle, personal and familial medical history, smoking status and alcohol consumption. The participants were classified as non-, ex-, and current drinkers and never-, ex-, and current smokers based on their response to lifetime alcohol consumption and smoking status on the questionnaire.

All volunteers underwent fasting blood collection. A radioimmunoassay method (DiaSorin, Stillwater, MN, USA) was used to determine serum 25(OH)D levels. The intra- and interassay coefficients of variation (CVs) were 5.6 and 10%, respectively. An enzymatic colorimetric method (ADVIA 1650, Bayer Ltd., Tokyo, Japan) was used to measure plasma levels of total calcium (CaT), with intra- and interassay CVs of 1.30 and 1.95%, respectively.

Blood samples were collected after a 10-h fast. Hemoglobin, serum creatinine (Scr), blood urea nitrogen, uric acid, albumin, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting blood glucose (FBG), calcium, and phosphorus levels were measured using an automatic biochemistry analyzer (Bayer ADVIA 1650). High sensitivity C-reactive protein (hs-CRP) level was measured by rate nephelometry (Beckman Array 360 System, Deerfield, IL, USA). Estimated GFR (eGFR) was calculated using a MDRD-4 (Modification of Diet in Renal Disease Study) formula: 186 × (Scr/88.4)^-1.154 × age^-0.203 × (0.742 if female) [16 (link)].