Sc 101237

Manufactured by Santa Cruz Biotechnology

Sc-101237 is a laboratory equipment product offered by Santa Cruz Biotechnology. The core function of this product is to facilitate sample processing and analysis in life science research applications. No further details are available at this time.

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Lab products found in correlation

Polyvinylidene fluoride (pvdf), by Thermo Fisher Scientific (2 mentions) Protease inhibitor, by Thermo Fisher Scientific (2 mentions) Bca assay, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (2 mentions) Sc 5275, by Santa Cruz Biotechnology (2 mentions)

2 protocols using sc 101237

Western Blot Analysis of Exosomal Proteins

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Exosomes were lysed in radio-immunoprecipitation assay buffer containing protease inhibitors (Thermo Scientific) and protein concentration quantified using the bicinchoninic acid assay (BCA assay, Thermo Scientific). Protein lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE), transferred onto polyvinylidene fluoride membranes (PVDF, Invitrogen) and immunoblotted with antibodies against EGFR (D38B1, Cell Signaling), CD63 (sc-5275, Santa Cruz), MGMT (MT3.1, EMD/Millipore), APNG (sc-101237, Santa Cruz) and GAPDH (14C10, Cell Signaling). All antibodies were used at 1,000-fold dilution (Cell Signaling) or at a concentration of 1 μg ml⁻¹ (Santa Cruz and EMD/Millipore). Following incubation with a horseradish peroxidase-conjugated secondary antibody (Cell Signaling), chemiluminescence was used for immunodetection (Thermo Scientific). Full images are presented in Supplementary Figs 4 and 8.

Shao H., Chung J., Lee K., Balaj L., Min C., Carter B.S., Hochberg F.H., Breakefield X.O., Lee H, & Weissleder R. (2015). Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma. Nature Communications, 6, 6999.

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Exosome Protein Characterization by Immunoblotting

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Exosomes were lysed in radio-immunoprecipitation assay buffer containing protease inhibitors (Thermo Scientific) and protein concentration quantified using the bicinchoninic acid assay (BCA assay, Thermo Scientific). Protein lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto polyvinylidene fluoride membranes (PVDF, Invitrogen) and immunoblotted with antibodies against EGFR (D38B1, Cell Signaling), CD63 (sc-5275, Santa Cruz), MGMT (MT3.1, EMD/Millipore), APNG (sc-101237, Santa Cruz) and GAPDH (14C10, Cell Signaling). All antibodies were used at 1000-fold dilution (Cell Signaling) or at a concentration of 1 µg/mL (Santa Cruz and EMD/Millipore). Following incubation with horseradish peroxidase-conjugated secondary antibody (Cell Signaling), chemiluminescence was used for immunodetection (Thermo Scientific). Full images are presented in Supplementary Figs. 4 and 8.

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