RIPA buffer containing Protease Inhibitor Cocktail (Selleck, B14001) was used for cell lysis. After centrifuging the cell extracts at 12,000 × g for 15 min, the supernatant was collected. Approximately 40 µg of total proteins were separated by SDS-polyacrylamide gel electrophoresis before being transferred to nitrocellulose (NC) membranes. Following a 2-h blocking step with 5% defatted milk at room temperature, the membranes were subsequently probed with primary antibodies. Subsequently, the membranes were washed three times and further probed with horseradish peroxidase (HRP)-labeled secondary antibodies (diluted at 1:4000) for 1 h at room temperature, and protein bands were visualized using SuperSignal™ West Pico Plus Chemiluminescent Substrate (ThermoFisher Scientific, TL275133). The primary antibodies used were as follows: anti-TAB182 (Santa Cruz, sc-514517, 1:1000), anti-LDHA (Cell Signaling Technology, 3582, 1:1000), anti-LDHB (Proteintech, 14824–1-AP, 1:1000), anti-c-MYC (Cell Signaling Technology, 18583, 1:1000), anti-SP1 (Cell Signaling Technology, 9389, 1:1000), anti-α-tubulin (Santa Cruz, sc-69969, 1:1000), and anti-actin (Proteintech, 81115–1-RR, 1:1000). The enhanced chemiluminescent reagent (Thermo, MA, USA) was used to detect protein bands.
Anti ldhb
Manufactured by Proteintech
Sourced in China
Anti-LDHB is a primary antibody that specifically recognizes the LDHB (Lactate Dehydrogenase B) protein. LDHB is an enzyme involved in the conversion of lactate to pyruvate as part of the glycolytic pathway. This antibody can be used to detect and study the expression of LDHB in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescent reagent, by Thermo Fisher Scientific (1 mentions)
Anti c myc, by Cell Signaling Technology (1 mentions)
Anti sp1, by Cell Signaling Technology (1 mentions)
Protease inhibitor cocktail, by Selleck Chemicals (1 mentions)
Anti actin, by Proteintech (1 mentions)
Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Anti α tubulin, by Santa Cruz Biotechnology (1 mentions)
Anti ldha, by Cell Signaling Technology (1 mentions)
Lactate dehydrogenase assay kit, by Nanjing Jiancheng (1 mentions)
Bilirubin, by Tokyo Chemical Industry (1 mentions)
Anti cd31 antibody, by Wuhan Servicebio Technology (1 mentions)
Anti hif 1α antibody, by Abcam (1 mentions)
Dcfh da, by Meilun (1 mentions)
N n diisopropylcarbodiimide, by Tokyo Chemical Industry (1 mentions)
Chlorin e6, by J&K Scientific (1 mentions)
Anti c myc antibody, by Proteintech (1 mentions)
Cou 6, by Merck Group (1 mentions)
Anti ldha, by Wuhan Servicebio Technology (1 mentions)
Lactate detection kit, by Nanjing Jiancheng (1 mentions)
2 protocols using anti ldhb
1
Western Blot Analysis of Cellular Proteins
2
Chlorin e6-Mediated Photodynamic Therapy
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Chlorin e6 was purchased from J&K Scientific Ltd. (Beijing, China). Diclofenac, morpholin-4-yl-acetic acid hydrochloride, 1-(3-dimethylaminopropyl)-3 ethylcarbodiimide hydrochloride (EDC), 4-dimethylaminopyridine (DMAP) and trimethylamine (TEA) were purchased from Aladdin (Shanghai, China). Bilirubin and N,N′-diisopropylcarbodiimide (DIC) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). mPEG2k-NH2 and Fmoc-NH-PEG2k-NH2 were purchased from Ponsure Biotechnology (Shanghai, China). Cou-6 was obtained from Sigma–Aldrich (USA). DiD and DCFH-DA were obtained from Meilunbio (Dalian, China). The annexin V-fluoresceine isothiocyanate (FITC) apoptosis detection kit and calcium-AM were obtained from Yeason (Shanghai, China). Lactate detection kit and lactate dehydrogenase assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Naijing, China). Anti-HIF-1α antibody, Cy3 goat anti-rabbit IgG (H + L) and Alexa fluor 647 goat anti-rabbit IgG (H + L) secondary antibody were purchased from Abcam (Hongkong, China). Anti-LDHA and anti-CD31 antibody were purchased from Servicebio Company (Wuhan, China). Anti-LDHB and anti-c-MYC antibody were purchased from Proteintech (Wuhan, China). Anti-VEGFα and anti-angiopoietin-2 antibody were purchased from Abclonal Company (Wuhan, China).
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