Anti calcineurin

Manufactured by Abcam

Sourced in United Kingdom

Anti-calcineurin is a lab equipment product that functions to inhibit the activity of the calcineurin enzyme. Calcineurin is a calcium-dependent serine/threonine protein phosphatase that plays a crucial role in various cellular processes. This product can be utilized in research settings to study the effects of calcineurin inhibition on various biological systems.

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Lab products found in correlation

Bca kit, by Thermo Fisher Scientific (3 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions) Anti nephrin, by Santa Cruz Biotechnology (1 mentions) Anti talin 1, by Abcam (1 mentions) Anti trpc6, by Santa Cruz Biotechnology (1 mentions) Anti calpain 1, by Abcam (1 mentions) Gapdh, by Abcam (1 mentions) Protease inhibitors cocktail, by Roche (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti pakt t308, by Cell Signaling Technology (1 mentions)

2 protocols using anti calcineurin

Western Blot Analysis of Kidney Proteins

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Total protein samples were extracted from cultured cells and kidney tissues. Protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). Samples were separated on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred onto a polyvinylidene difluoride membrane. The membrane was then blocked overnight at 4 °C with primary antibodies, namely anti-TRPC6 (Santa Cruz, USA), anti-calpain-1 (Abcam, UK), anti-Talin-1 (Abcam), anti-nephrin (Santa Cruz) and anti- calcineurin (Abcam). After washing, the membrane was incubated with goat anti-rabbit IgG (H + L; Jackson ImmunoResearch Laboratories, USA). The membrane was also processed to detect GAPDH (Abcam) for equal loading. The bands recognized by the primary antibody were visualized, and the optical densities of the protein bands were analyzed using TanonImage software (Tanon, China).

Ding Y., Tang X., Wang Y., Yu D., Zhu C, & Yu J. (2021). Tetrandrine alleviates podocyte injury via calcium-dependent calpain-1 signaling blockade. BMC Complementary Medicine and Therapies, 21, 296.

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YZ129 Modulates AKT and Calcineurin

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U87 cells were treated with 0.25 μM, 5 μM YZ129 or DMSO (as control) for 24 h. Cells were washed with PBS three times and lysed on ice with RIPA buffer containing protease inhibitors cocktails (Roche) for 30 min. The lysate was centrifuged at 12,000×g for 10 min at 4 °C and the protein content of the supernatant was quantified by using the BCA kit (Thermo Scientific). The lysate was separated on a 4–15% gel (Bio-Rad), transferred to PVDF membranes and probed with antibodies against different proteins (anti-AKT, Cell Signaling Technology, Cat No. #9272; anti-pAKT-T308, Cell Signaling Technology, #4056; anti-calcineurin, Abcam, #ab109412).

Liu Z., Li H., He L., Xiang Y., Tian C., Li C., Tan P., Jing J., Tian Y., Du L., Huang Y., Han L., Li M, & Zhou Y. (2019). Discovery of small molecule inhibitors of the HSP90-calcineurin-NFAT pathway against glioblastoma. Cell chemical biology, 26(3), 352-365.e7.

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