8 cyclopentyl 1 3 dipropylxanthine

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8-cyclopentyl-1,3-dipropylxanthine is a chemical compound used in laboratory research. It functions as an adenosine receptor antagonist.

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3 protocols using 8 cyclopentyl 1 3 dipropylxanthine

Adenosine Receptor Pharmacology Protocols

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Adenosine, 2-chloroAdenosine (stable Adenosine analogue), and erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA; increases endogenous Adenosine by inhibiting Adenosine deaminase and thus reducing the metabolism of Adenosine to inosine) were purchased from Sigma-Aldrich (St. Louis, MO). N⁶-cyclopentylAdenosine (CPA; selective A₁ receptor agonist), CGS21680 (selective A_2A receptor agonist), 8-cyclopentyl-1,3-dipropylxanthine (DPCPX; selective A₁ receptor antagonist), 5-iodotubercidin (IDO; increases endogenous Adenosine by inhibiting Adenosine kinase and thus reducing the metabolism of Adenosine to 5’-AMP), 5’-N-ethylcarboxamidoAdenosine (NECA; non-selective Adenosine receptor agonist), 5’-N-methylcarboxamidoAdenosine (MECA; non-selective Adenosine receptor agonist), 1-deoxy-1-[6-[[(3-iodophenyl)methyl]amino]-9H-purin-9-yl]-N-methyl-β-D-ribofuranuronamide (IB-MECA; selective A₃ receptor agonist), SCH442416 (selective A_2A receptor antagonist), MRS1754 (selective A_2B receptor antagonist), VUF5574 (selective A₃ receptor antagonist) were purchased from Tocris (Minneapolis, MN). ³H-thymidine (specific activity, 11.8 Ci/mmol) was purchased from PerkinElmer NEN (Walthan, MA). All other reagents were of tissue culture or best grade available.

Dubey R.K., Fingerle J., Gillespie D.G., Mi Z., Rosselli M., Imthurn B, & Jackson E.K. (2015). Adenosine Attenuates Human Coronary Artery Smooth Muscle Cell Proliferation By Inhibiting Multiple Signaling Pathways that Converge on Cyclin D. Hypertension, 66(6), 1207-1219.

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Pharmacological Modulation of Adenosine Signaling

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(R)-(+)-[2,3-Dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenylmethanone mesylate (WIN55212-2), 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), N6-cyclopentyladenosine (CPA), N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251), rolipram, and forskolin were purchased from Tocris (Bristol, UK). Adenosine deaminase was obtained from Sigma-Aldrich. Stock solutions of WIN55212-2 (20 mM), rolipram (20 mM), DPCPX (50 μM), and AM251 (5 mM) were prepared in dimethyl sulfoxide (DMSO). forskolin (50 mM) and CPA (2 mM) stock solutions were prepared in ethanol and water, respectively. Suitable dilutions of each stock solution with Krebs buffer were made before performing the experiments.

Serpa A., Correia S., Ribeiro J.A., Sebastião A.M, & Cascalheira J.F. (2015). The Combined Inhibitory Effect of the Adenosine A1 and Cannabinoid CB1 Receptors on cAMP Accumulation in the Hippocampus Is Additive and Independent of A1 Receptor Desensitization. BioMed Research International, 2015, 872684.

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Effects of Adenosine A1 Receptor Antagonist on Amphetamine and MK-801 Behavioral Responses

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d-Amphetamine hemisulfate salt (Sigma-Aldrich, Saint Louis, USA) and MK-801 (dizocilpine, Tocris Bioscience, Bristol, UK) were dissolved in physiological saline and administered at doses of 1 mg/kg sc, and 0.3 mg/kg ip, respectively. 5′-Chloro-5′-deoxy-N⁶-( ±)-(endo-norborn-2-yl)adenosine (5′-Cl-5′-deoxy-ENBA, Tocris Bioscience, Bristol, UK) was dissolved in 0.5% DMSO in physiological saline and administered at a dose of 0.1 mg/kg ip 30 min before amphetamine or MK-801. 8-Cyclopentyl-1,3-dipropylxanthine, a selective antagonist of adenosine A₁ receptors (DPCPX, Tocris Bioscience, Bristol, UK) [16 (link)] was dissolved in 10% DMSO in physiological saline and administered at doses of 1 or 2 mg/kg ip 10 min before 5′-Cl-5′-deoxy-ENBA (40 min before amphetamine or MK-801). Physiological saline was used as the control for amphetamine and MK-801, 0.5% DMSO for 5′-Cl-5′-deoxy-ENBA and 10% DMSO for DPCPX (Fig. 1).Fig. 1

A description of groups of rats and time schedule of the experiment. 5'Cl-ENBA 5'-Cl-5'-deoxy-ENBA, Amph amphetamine, MK MK-801 (dizocilpine), SOLV solvent

Ossowska K., Kosmowska B, & Wardas J. (2020). Potential antipsychotic action of the selective agonist of adenosine A1 receptors, 5′-Cl-5′-deoxy-ENBA, in amphetamine and MK-801 rat models. Pharmacological Reports, 72(3), 580-588.

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