Ab65200

Mouse anti-HA (MMS-101P, Biolegend, San Diego, CA), mouse anti-phospho tyrosine (PY72) (P172.1, InVivo Biotech Services, Henningsdorf, Germany), rabbit anti-c-Cbl (sc-170, Santa Cruz Biotechnologies, Heidelberg, Germany), mouse monoclonal anti-α-Tubulin (Sigma Aldrich, St. Louis, MO); living colors rabbit anti-GFP (632593, Clontech, Mountain View, CA), living colors mouse anti-GFP (632681, Clontech, Mountain View, CA), mouse anti-Rab11 (610656, BD Biosciences, Heidelberg, Germany), rabbit anti-Rab11a (ab65200, Abcam), rabbit anti-Rab11 (3539, Cell Signaling Technology, Danvers, MA), rabbit EGFR (4267, Cell Signaling Technology, Danvers, MA), rabbit pY1045 (2237, Cell Signaling Technology, Danvers, MA), rabbit pY1068 (3777, Cell Signaling Technology, Danvers, MA), goat EGFR (AF231, R&D Systems, Minneapolis, MN), mouse pY845 (558381, BD Biosciences, Heidelberg, Germany), mouse anti-Rab5 (610281, BD Biosciences, Heidelberg, Germany), rabbit anti-phospho ERK-1/2 Thr/Tyr 202/204 (9101, Cell Signaling Technology, Danvers, MA), mouse anti-ERK1/2 (Ab366991, Abcam); rabbit anti phosphor-Akt Ser473 (9271, Cell Signaling Technology, Danvers, MA), mouse anti-Akt (pan) (2920, Cell Signaling Technology, Danvers, MA).

Protein lysates isolated from orange adductor muscles and white adductor muscles were used for Western blot analysis as described by Funabara et al. [43 (link)]. Proteins were separated through SDS-PAGE and subsequently transferred to polyvinylidene fluoride membranes. The membranes were incubated overnight at 4 °C with primary antibodies. Tubulin was selected as the internal control. The primary antibodies were anti-tubulin (Abcam, ab6160, USA), anti-RAB11A (Abcam, ab65200, USA), anti-VPS (Abcam, ab98929, USA) and anti-TIF (abcam, ab230321, USA). Then, the membranes were rinsed thrice with TBS and were incubated with secondary antibodies (Beyotime, A0208) for 2 h at 37 °C. Gel documentation was observed using AlphaImager® HP (ProteinSimple, 92–13,824-00, USA). Band intensity was quantified with IPWIN software.