Gould p23db

After rats were anesthetized with sodium pentobarbital (45 mg/kg i.p.), the hearts were rapidly excised and perfused with Krebs‐Henseleit (K‐H) solution at 37°C using a Langendorff apparatus at a constant pressure of 80 mm Hg as previously described. A water‐filled latex balloon connected to a pressure transducer (Gould P23Db, AD Instrument) was inserted into the LV cavity to achieve a stable LVEDP of 5‐10 mm Hg during initial equilibration. After equilibration perfusion, the heart was subjected to 30 minutes of global no‐flow ischaemia followed by 45 minutes of reperfusion. LVDP and ±dp/dt max were evaluated with the PowerLab system (AD Instrument).

The rats were anesthetized with sodium pentobarbital (66 mg/kg, i.p.), and their hearts were quickly removed and retrogradely perfused through the aorta on a Langendorff apparatus with Krebs–Henseleit (K–H) buffer at constant pressure (76 mmHg) and temperature (37 °C). The ingredients of K-H buffer were (in mmol/L): NaCl 118, KCl 4.7, MgSO₄ 1.2, CaCl₂ 2.5, KH₂PO₄ 1.2, NaHCO₃ 25 and glucose 11 (continuously bubbled with 95% O₂ and 5% CO₂, pH 7.4). A latex balloon-tipped catheter filled with saline was placed into the left ventricle through the left atrium and adjusted to a left ventricular end diastolic pressure (LVEDP) of 5–10 mmHg during the initial equilibration. The distal end of the catheter was connected to a pressure transducer (model Gould P23Db, AD Instrument Ltd., Australia) and the pressure signal was recorded with PowerLab system (AD Instrument). Left ventricular developed pressure (LVDP), LVEDP and the maximal differentials of LVDP (±LVdp/dt_max) were continuously recorded. After 30 min stabilization with K-H buffer, the heart was subjected to 30 min no-flow ischemia, followed by 60 min reperfusion (or 120 min reperfusion for the determination of infarct size). The data were analyzed by using Chart software (AD Instrument).