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Technai g2 spirit biotwin

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The Technai G2 Spirit BioTwin is a transmission electron microscope (TEM) designed for biological applications. It provides high-resolution imaging capabilities for the analysis of biological samples.

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G2 Spirit BioTWIN (18 citations) Technai G2 (8 citations) Technai G2 Spirit (5 citations) Spirit G2 (4 citations) Technai G2 Spirit Biotwin transmission electron microscope (3 citations) BioTwin Spirit (2 citations)

2 protocols using technai g2 spirit biotwin

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Negative Stain GroEL TEM Reconstruction

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TEM studies for the reconstruction of negatively stained GroEL were performed at ambient temperature on a Technai G2 Spirit BioTwin (Thermo Fisher Scientific) fitted with a NanoSprint15 MK-II 15 Mpix camera (AMT Imaging), operated at 120 kV. Microscope and camera control software included Tecnai version 3.1.3 and AMT Capture Engine version 7.00 respectively. For the 3D reconstruction, a defocused image series ranging from 0.1 µm to 2 µm in 0.1 µm steps were collected using the SerialEM software package version 3.8.7 (https://bio3d.colorado.edu/SerialEM/). Collection conditions are summarized in Supplementary Table 1. Particle picking, classification, reconstruction and refinement of 3D maps were performed in cisTEM version 1 (https://cistem.org/). Model fitting, imaging and figure creation were done using UCSF Chimera version 1.16 (https://www.cgl.ucsf.edu/chimera/, Supplementary Fig. 4).
Westphall M.S., Lee K.W., Salome A.Z., Lodge J.M., Grant T, & Coon J.J. (2022). Three-dimensional structure determination of protein complexes using matrix-landing mass spectrometry. Nature Communications, 13, 2276.
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2

Structural Analysis of Macromolecular Complexes

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Imaging and 3D reconstructions of negatively stained GroEL, β-Galactosidase, and 20S Proteasome were performed as previously described.14 (link) Briefly, images were collected on a Technai G2 Spirit BioTwin (Thermo Fisher Scientific) defoci ranging from 0.5μm to 2μm using SerialEM software version 3.8.7.(Acquisition settings summarized in Supplementary Table 1.) and 3D reconstruction was performed using cis-TEM (https://cistem.org/). Model fitting and figure creation was performed on UCSF ChimeraX version 1.3 (https://www.cgl.ucsf.edu/chimerax/).
Salome A.Z., Lee K.W., Grant T., Westphall M.S, & Coon J.J. (2022). Matrix-Landing Mass Spectrometry for Electron Microscopy Imaging of Native Protein Complexes. Analytical chemistry, 94(50), 17616-17624.
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