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3 protocols using calcium nitrite

1

Analytical Chemistry Reagents Protocol

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LC-MS-grade methanol, formic acid, water, acetonitrile, sodium chloride, potassium chloride, and magnesium sulfate were from Fisher Scientific (Fair Lawn, NJ). Calcium nitrite, cysteine, Trizma hydrochloride, and Trizma base were from Sigma-Aldrich (Saint Louis, MO). Acetylcholine and amino acid standards were purchased at reagent grade or higher purity from Acros Organics (Fair Lawn, NJ).
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2

LC-MS Metabolite Analysis Workflow

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All compounds, including LC-MS grade acetonitrile, methanol, and water were purchased from Fisher Scientific (Fair Lawn, NJ) unless otherwise noted. Calcium nitrite, cysteine, Trizma hydrochloride, and Trizma base were from Sigma Aldrich (Saint Louis, MO).
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3

Fluorescent Dextran Labeling Protocol

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Unless otherwise noted, LC-MS grade solvents (for HRMS), reagent-grade chemicals, TPCK-modified trypsin (for HRMS), and Alexa Fluor 488 (green fluorescent dextran) were purchased from Fisher Scientific (Pittsburgh, PA). Calcium nitrite, cysteine, Trizma hydrochloride, and Trizma base were purchased from Sigma-Aldrich (St. Louis, MO).
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