Anti cortactin ab 33333

Manufactured by Abcam

Anti-cortactin (ab-33333) is a protein-specific antibody that recognizes cortactin, an important regulator of the actin cytoskeleton. This antibody can be used to detect and study cortactin in various experimental systems.

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Lab products found in correlation

Anti arp2 h 84 sc 15389, by Santa Cruz Biotechnology (2 mentions) Anti tks5 fish m 300 sc 30122, by Santa Cruz Biotechnology (2 mentions) Rhodamine labeled phalloidin, by Thermo Fisher Scientific (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Mmpsense 645 fast fluorescent imaging agent, by PerkinElmer (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Anti cd31, by BD (1 mentions) Anti pcna, by Abcam (1 mentions) Goat anti rabbit 800cw, by LI COR (1 mentions) Goat anti mouse 680lt, by LI COR (1 mentions) Anti py397 fak, by Thermo Fisher Scientific (1 mentions) Alexa fluor conjugated secondary, by Thermo Fisher Scientific (1 mentions) Anti phosphotyrosine clone 4g10 05 321, by Merck Group (1 mentions) Anti fak clone 77 610088, by BD (1 mentions) Anti gfp clones 7 1 and 13, by Roche (1 mentions) Anti β actin clone ac 15 a5441, by Merck Group (1 mentions)

2 protocols using anti cortactin ab 33333

Immunofluorescence Staining of Cortactin

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For immunofluorescence, anti-cortactin (ab-33333) and anti-PCNA were obtained from Abcam; anti-Arp2 (H-84) (SC-15389) and anti-Tks5 (FISH M-300) (SC-30122) were obtained from Santa Cruz Biotechnology; anti-pY421-cortactin (C0739) was obtained from Sigma-Aldrich; anti-CD31 was obtained from BD Biosciences; anti-cleaved caspase 3 was obtained from Cell Signaling Technology. Rhodamine-labeled phalloidin and Alexa Fluor-conjugated secondary antibodies were obtained from Molecular Probes (Thermo Fisher Scientific). MMP Sense 645 FAST fluorescent imaging agent (NEV10100) was obtained from Perkin-Elmer. Imatinib mesylate (STI-571) (HY-50946), nilotinib (HY-10159), and GNF-5 (HY-15738) were purchased from MedChem Express.

Meirson T., Genna A., Lukic N., Makhnii T., Alter J., Sharma V.P., Wang Y., Samson A.O., Condeelis J.S, & Gil-Henn H. (2018). Targeting invadopodia-mediated breast cancer metastasis by using ABL kinase inhibitors. Oncotarget, 9(31), 22158-22183.

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Immunofluorescence and Western Blotting Assay

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For immunofluorescence, anti-cortactin (ab-33333) was obtained from Abcam; anti–pY402-Pyk2 and anti–pY397-FAK were obtained from Invitrogen; anti-Arp2 (H-84; SC-15389) and anti-Tks5 (FISH M-300; SC-30122) were obtained from Santa Cruz Biotechnology, Inc.; and antivinculin (clone hVIN1; V9131), anti–pY421-cortactin (C0739), and anti–pY466-cortactin (C0864) were obtained from Sigma-Aldrich. Rhodamine-labeled phalloidin and Alexa Fluor–conjugated secondary antibodies were obtained from Molecular Probes. For Western blotting, anticortactin (clone 4F11; 05-180) and antiphosphotyrosine (clone 4G10; 05-321) were obtained from EMD Millipore; anti-Pyk2 (3480 and 3292) was obtained from Cell Signaling Technology; anti-FAK (clone 77; 610088) was obtained from BD; anti-GFP (clones 7.1 and 13.1; 11814460001) was obtained from Roche; and anti–β-actin (clone AC-15; A5441) was obtained from Sigma-Aldrich. Secondary antibodies (goat anti–mouse 680LT and goat anti–rabbit 800CW) were obtained from LI-COR Biosciences.

Genna A., Lapetina S., Lukic N., Twafra S., Meirson T., Sharma V.P., Condeelis J.S, & Gil-Henn H. (2018). Pyk2 and FAK differentially regulate invadopodia formation and function in breast cancer cells. The Journal of Cell Biology, 217(1), 375-395.

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