Sb206553

Manufactured by Merck Group

Sourced in Germany

SB206553 is a pharmaceutical compound produced by Merck Group. It is a synthetic small molecule that acts as a selective antagonist for the 5-HT6 serotonin receptor. The compound is primarily used in research settings to study the role of the 5-HT6 receptor in various biological processes.

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4 protocols using sb206553

Cellular Signaling Assay Reagents

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5-HT hydrochloride, HTR antagonists Ketanserin, SB206553, Collagenase Type IV, Collagenase P (Roche), formic acid (puriss. p.a.), and Dexamethasone were obtained from Sigma-Aldrich (Steinheim, Germany). Fura-2 LeakRes (AM) was purchased from Teflabs (Austin, TX, USA), William’s Medium E cell culture media from PAN Biotech (Aidenbach, Germany), and FCS from Gibco (Life Technologies, Darmstadt, Germany). Furthermore, RIPA Lysis Buffer was obtained from Thermo Scientific (Waltham, MA, USA), insulin (Insuman Basal) from Sanofi (Frankfurt, Germany), acetonitrile (HPLC-MS grade) from Wicom (Heppenheim, Germany), and 5-HT-d4 (hydrochloride) from Cayman Chemicals (Ann Arbor, MI, USA). All other reagents were purchased from Merck (Darmstadt, Germany), Carl Roth (Karlsruhe, Germany), or PanReac AppliChem (Darmstadt, Germany).

Asuaje Pfeifer M., Liebmann M., Beuerle T., Grupe K, & Scherneck S. (2022). Role of Serotonin (5-HT) in GDM Prediction Considering Islet and Liver Interplay in Prediabetic Mice during Gestation. International Journal of Molecular Sciences, 23(12), 6434.

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Pharmacological Modulation of BACE1 and APP Processing

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Fluoxetine, TAPI-1, SB271046, SB742457, L-685,458 and roscovitine were purchased from Selleck Chemicals. Amitriptyline hydrochloride, ST1936 and SB258585 were from Tocris Bioscience. Protriptyline hydrochloride, amoxapine, trimipramine maleate, SB215505, SB206553, cAMP, L-ascorbic acid and DAPI were purchased from Sigma. Escitalopram oxalate was purchased from Lundbeck. Sertraline hydrochloride was from Pfizer. BACE inhibitor IV (BSI IV) was from Calbiochem. Recombinant human BDNF, GNDF, and IGF-I were from Peprotech. CellTiter-Glo was from Promega. Immunoblotting was performed with the following antibodies: anti-ADAM10 (Ab1997, Abcam), anti-BACE1 N-termimus (AP7774b, Abgent), anti-APP-CTF (A8717, Sigma), anti-actin (A2066, Sigma), anti-CDK5 (sc-173, Santa Cruz), and Rabbit anti-β-arrestin-1/2 (A1CT) antibody was a kind gift from Dr. Robert J. Lefkowitz. Immunofluorescence staining was performed with the following primary antibodies: anti-Tuj1 (801201, BioLegend), anti-Sox2 (sc-17320, Santa Cruz), anti-Map2 (Ab5622, Millipore), and anti-GFAP (sc-6170, Santa Cruz).

Li X., Wang Q., Hu T., Wang Y., Zhao J., Lu J, & Pei G. (2017). A tricyclic antidepressant, amoxapine, reduces amyloid-β generation through multiple serotonin receptor 6-mediated targets. Scientific Reports, 7, 4983.

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Miniosmotic Pump Implantation and Agonist/Antagonist Delivery

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Miniosmotic pumps (Alzet 1007D) were assembled under sterile conditions, filled with vehicle (saline), fenfluramine (200 µg/ml) (Sigma), RO600175 (400 µg/ml) (Tocris Bioscience) or SB206553 (160 µg/ml) (Sigma) and allowed to equilibrate at 37°C overnigh t. Pump implantation was performed on a stereotaxic rig as previously described (Ihrie et al., 2011 (link)), such that the cannula was inserted into the lateral ventricle (x: 1.10, y: 0.00, z: −2.35). Mice with fenfluramine pumps were analyzed 2 days post-implantation and mice with RO600175 or SB206553 pumps were analyzed 5 days post-implantation. For acute agonist and antagonist treatment, 500 nl of saline, RO600175 (400 µg/ml) or SB206553 (160 µg/ml) was injected into the lateral ventricle (x: 0.63, y: 0.38, z: −2.40) as described above (see Axon Tracing). V-SVZ wholemounts were dissected from the contralateral hemispheres 4 hours post-injection. The mice were injected I.P. with 50 mg/kg BrdU 1 hour before perfusion.

Tong C.K., Chen J., Cebrián-Silla A., Mirzadeh Z., Obernier K., Guinto C.D., Tecott L.H., García-Verdugo J.M., Kriegstein A, & Alvarez-Buylla A. (2014). Axonal Control of the Adult Neural Stem Cell Niche. Cell stem cell, 14(4), 500-511.

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Synthesis and Pharmacological Evaluation of o-PIT

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o-phenyl-3-iodotyramine hydrochloride (o-PIT) was synthesized by Servier chemists. Raclopride, RX821,002, WAY100,635, MDL100907 and SB206553 were purchased from Sigma. Compounds were dissolved in sterile water. MDL100907 and SB206553 were dissolved in sterile water plus lactic acid, and the pH was adjusted near neutrality. Sterile water with or without lactic acid were used as vehicle control (VEH). All solutions were injected in a volume of 10 mL/kg. Dose was expressed in terms of free base.

Mantas I., Millan M.J., Di Cara B., Groenink L., Veiga S., Cistarelli L., Brocco M., Bertrand M., Svenningsson P, & Zhang X. (2021). Trace Amine-Associated Receptor 1 Contributes to Diverse Functional Actions of O-Phenyl-Iodotyramine in Mice but Not to the Effects of Monoamine-Based Antidepressants. International Journal of Molecular Sciences, 22(16), 8907.

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