Fetal bovine serum (FBS) and cell culture medium were purchased from HyClone. MG132 and kanamycin were purchased from Sigma. JNK IN8 was obtained from MedChem Express. Plasmids for overexpressing HMGA1 or GRP75 and HMGA1‐specific short hairpin RNA (shRNA) were designed and synthesized by GeneChem. Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio. A cell counting kit‐8 (CCK8) kit was purchased from Bioss. A migration assay kit was obtained from BD Biosciences. Cell invasion assay chambers (24 wells) were purchased from Corning. Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P‐JNK (Thr183/Tyr185), c‐JUN, P‐c‐JUN (Ser63), P‐c‐JUN (Ser73), and GAPDH were purchased from Cell Signaling Technology. A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scientific.
Small interfering rna sirna oligonucleotides
Manufactured by RiboBio
Sourced in China
Small interfering RNA (siRNA) oligonucleotides are synthetic, double-stranded RNA molecules designed to target and silence specific gene expression. They function by binding to and degrading complementary mRNA, thereby preventing the translation of the target gene.
Automatically generated - may contain errors
Lab products found in correlation
Migration assay kit, by BD (4 mentions)
Ubiquitin, by Cell Signaling Technology (4 mentions)
Jnk in 8, by MedChemExpress (4 mentions)
A cell counting kit 8 kit, by Bioss Antibodies (4 mentions)
Cell invasion assay chambers 24 wells, by Corning (4 mentions)
Kanamycin, by Merck Group (4 mentions)
Mg132, by Merck Group (4 mentions)
Gapdh, by Cell Signaling Technology (4 mentions)
Cycloheximide chx and primary antibodies against, by Cell Signaling Technology (3 mentions)
P c jun ser63, by Cell Signaling Technology (3 mentions)
P c jun ser73, by Cell Signaling Technology (3 mentions)
Cycloheximide (chx), by Cell Signaling Technology (1 mentions)
Pierce co immunoprecipitation kit, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Short hairpin rna shrna, by Genechem (1 mentions)
5 protocols using small interfering rna sirna oligonucleotides
1
Investigating HMGA1-GRP75 Interaction in Cancer Progression
2
Modulating circRNA and IGF2BP3 Expression
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RiboBio (Guangzhou, China) synthesized recombinant lentivirus containing short hairpin RNA targeting circ_0053943 (sh-circ_0053943#1/2/3) and IGF2BP3 (sh-IGF2BP3#1/2), along with the full-length targeting sequence of circ_0053943 and IGF2BP3, including the corresponding negative control. Obio (Shanghai, China) synthesized the IGF2BP3 full-length (FL) and truncation-mutation plasmids with a C-terminus 3× Flag tag. Small interfering RNA (siRNA) oligonucleotides targeting methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) were also synthesized by RiboBio. Transfection of short hairpin (shRNAs) and plasmid vectors was carried out using Lipofectamine 3000 (Invitrogen). The transfection efficiency was subsequently confirmed through qRT-PCR. Detailed sequences of shRNAs and siRNAs (for silencing) can be found in Suppl. Table 3.
3
Inhibition of HMGA1 and GRP75 in Cancer
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Fetal bovine serum (FBS) and cell culture medium were purchased from HyClone (South Logan, UT, USA). MG132 and kanamycin were purchased from Sigma (St. Louis, MO, USA). JNKIN8 was obtained from MedChem Express (Shanghai, China). Plasmids for overexpressing HMGA1 or GRP75 and HMGA1speci c short hairpin RNA (shRNA) were designed and synthesized by GeneChem (Shanghai, China).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
4
Inhibition of HMGA1 and GRP75 in Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal bovine serum (FBS) and cell culture medium were purchased from HyClone (South Logan, UT, USA). MG132 and kanamycin were purchased from Sigma (St. Louis, MO, USA). JNKIN8 was obtained from MedChem Express (Shanghai, China). Plasmids for overexpressing HMGA1 or GRP75 and HMGA1speci c short hairpin RNA (shRNA) were designed and synthesized by GeneChem (Shanghai, China).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
5
Investigating HMGA1 and GRP75 Interactions
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+ Expand
Fetal bovine serum (FBS) and cell culture medium were purchased from HyClone (South Logan, UT, USA). MG132 and kanamycin were purchased from Sigma (St. Louis, MO, USA). JNK IN8 was obtained from MedChem Express (Shanghai, China). Plasmids for overexpressing HMGA1 or GRP75 and HMGA1speci c short hairpin RNA (shRNA) were designed and synthesized by GeneChem (Shanghai, China).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
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