Ramp2

Rat The ADM (molecular formula: C₂₄₂H₃₈₁N₇₇O₇₅S₅) was obtained from Bachem (Bubendorff, Switzerland). PA was purchased from Sigma-Aldrich. ADM receptor antagonist ADM22-52 and Akt inhibitor A6730 were purchased from Anaspec (Fremont, CA, USA). DMEM, 0.25% trypsin-EDTA, fetal bovine serum, trypsin, and streptomycin/penicillin were from Thermo Fisher Scientific (Pudong New District, Shanghai, China). The antibodies of ADM, CRLR, RAMP2, RAMP3, and TNF-α were obtained from Affinity Biosciences (Pottstown, PA, USA). The antibodies of IL-6, IL-1β, SOD2, GPx1, CAT, and GAPDH were from Proteintech (SANYING, Wuhan, China). Total Akt and phosphorylated Akt were purchased from Cell Signaling Technology (Shanghai, China).

Rat ADM (molecular formula: C₂₄₂H₃₈₁N₇₇O₇₅S₅) was obtained from Bachem (Bubendorff, Switzerland). PA and Compound C were purchased from Sigma Aldrich (St. Louis, MO, USA). ADM22-52 was purchased from Anaspec (Fremont, CA, USA). DMEM, 0.25% trypsin-EDTA, fetal bovine serum, trypsin and streptomycin/penicillin were from Thermo Fisher Scientific (Waltham, MA, USA). The primary antibodies against α-actin and Runx2 were purchased from Abcam (Burlingame, CA, USA). ADM, CRLR, RAMP2, RAMP3, TNF-α and IL6 were obtained from Affinity Biosciences (Pottstown, PA, USA). The antibodies of IL-1β, NOX2, NOX4 and GAPDH were from Proteintech (SANYING, Wuhan, China).