Anti β catenin c2206

Manufactured by Merck Group

Sourced in United States

Anti-β-catenin (C2206) is a monoclonal antibody that targets the β-catenin protein. It is a laboratory reagent used in research applications to detect and study the expression and localization of β-catenin in biological samples.

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Lab products found in correlation

Anti wnt7a sc26360, by Santa Cruz Biotechnology (2 mentions) Anti β actin a3853, by Merck Group (2 mentions) Tissuelyser lt, by Qiagen (2 mentions) Anti cd45 30 f11, by BioLegend (1 mentions) Gtx121937, by GeneTex (1 mentions) Anti ki67 20raj1, by Thermo Fisher Scientific (1 mentions) Anti f4 80 bm8, by BioLegend (1 mentions) Anti ly6c hk1, by BioLegend (1 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) Clone jc70a, by Agilent Technologies (1 mentions) Anti cd31, by Agilent Technologies (1 mentions) 3 3 diaminobenzidine (dab), by Thermo Fisher Scientific (1 mentions) Anti pcna sc 56, by Santa Cruz Biotechnology (1 mentions) Bicinchoninic acid (bca), by Thermo Fisher Scientific (1 mentions) Protein g plus agarose, by Santa Cruz Biotechnology (1 mentions)

6 protocols using anti β catenin c2206

Comprehensive Immunostaining of Frozen Tissues

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Frozen skin tissue sections were incubated with different primary antibodies at 4 °C overnight; antibodies included anti-CD49f-biotin (GoH3, 1:150, BioLegend), anti-Ki67 (20Raj1, 1:100, eBioscience), anti-Akt (GTX28932, phospho Ser473, 1:150, GeneTex), anti-Akt (Akt 1+2+3) (GTX121937, 1:150, GeneTex), anti-CD34-biotin (RAM34, 1:150, eBioscience), anti-Lgr-5 (ab137484, 1:200, Abcam), anti-CD45 (30-F11, 1:150, BioLegend), anti-CD45-Biotin (30-F11, 1:150, BioLegend), anti-F4/80-Biotin (BM8, 1:150, BioLegend), anti-F4/80 (BM8, 1:150, BioLegend), anti-Ly6C-Biotin (RB6-8C5, 1:200, BioLegend), anti-Ly6C (HK1.4, 1:200, BioLegend), anti-MHC II (14-4-4S, 1:200, eBioscience), anti-TNF-α (1F3F3D4, 1:100, eBioscience), anti-TNFR1-Biotin (55R-170, 1:100, BioLegend), anti-β-catenin (C2206, 1:150, Sigma) and anti-Phospho-β-Catenin (Ser552) (5651S, 1:100, CST Signaling). Secondary antibodies with different fluorescence conjugates (FITC, Cy3/TRITC and Alex Fluor 647) were used for detection, and cells were visualized with an Olympus FV1000 confocal microscope.

Wang X., Chen H., Tian R., Zhang Y., Drutskaya M.S., Wang C., Ge J., Fan Z., Kong D., Wang X., Cai T., Zhou Y., Wang J., Wang J., Wang S., Qin Z., Jia H., Wu Y., Liu J., Nedospasov S.A., Tredget E.E., Lin M., Liu J., Jiang Y, & Wu Y. (2017). Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF. Nature Communications, 8, 14091.

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Immunohistochemical Analysis of PAR2-Induced Mammary Gland Tissue

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Tissue samples derived from PAR₂ induced mammary glands were fixed with 4% formaldehyde in PBS, embedded in paraffin, and sectioned (5-μm sections). After deparaffinization and rehydration, the sections were stained with H&E or subjected to immunohistochemistry. For this, the slides were incubated 3% H₂O₂ prior to antigen retrieval. Antigen unmasking was carried out heating (20 min) in a microwave oven in 10mM Tris buffer containing 1mM EDTA. After blocking slides were incubated with the following primary antibodies: anti β-catenin (C-2206, Sigma-Aldrich St Louis MO, USA), anti PCNA (sc-56, Santa Cruz Biotechnology, USA dilution 1:200) anti DVL1 (sc7397, Santa Cruz Biotechnology, Dallas Texas, USA; goat polyclonal IgG) or anti CD31 (Dako, Clone JC70A, Carpinteria, CA). Color was developed using the 3,3′-diaminobenzidine (DAB) (Thermo Scientific, Walham, MA, USA) or the Zymed AEC substrate kit (Zymed Laboratories So, San-Francisco, CA, USA), followed by counter staining with Mayer's haematoxylin. Controls without addition of primary antibodies showed low or no background staining in all cases.

Nag J.K., Kancharla A., Maoz M., Turm H., Agranovich D., Gupta C.L., Uziely B, & Bar-Shavit R. (2017). Low-density lipoprotein receptor-related protein 6 is a novel coreceptor of protease-activated receptor-2 in the dynamics of cancer-associated β-catenin stabilization. Oncotarget, 8(24), 38650-38667.

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Protein Extraction and Western Blotting

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Lung tissue protein extracts were obtained from mouse lungs homogenized in tissue protein extraction lysis buffer containing a cocktail of 1 M HEPES, 5 M NaCl, 10% Triton X-100, 1 M DTT, 0.5% EDTA, 20 mM NaVO₃, 10 mM PMSF, 0.5 M Na-β-glycerophosphate and protease inhibitors using Qiagen TissueLyser LT (Qiagen), while NSCLC cells were lysed in a lysis buffer (MAP kinase lysis buffer), and the western blotting analysis was carried out as previously described.^{18 (link), 19 (link)} Anti-β-actin (A3853) and anti-β-catenin (C2206) were purchased from SIGMA (Sigma-Aldrich, St Louis, MO, USA). Anti-Caspase 3 (9662S), anti-Skp2 (4313S), anti-p27^kip (2552S), anti-Cdk6 (3136S), anti-CyclinD1 (2922S), anti-LC3A/B (4108S) anti-p-pRB (3590S) were purchased from Cell Signaling (Cell Signaling Technology Inc., Danvers, MA, USA) and anti-Wnt7a (SC26360) was purchased from Santa Cruz Biotechnology. Densitometric analysis was performed using the ImageJ software (NIH, Bethesda, MD, USA). Band intensities were normalized to their corresponding controls and are represented in the figures.

Bikkavilli R.K., Avasarala S., Van Scoyk M., Arcaroli J., Brzezinski C., Zhang W., Edwards M.G., Rathinam M.K., Zhou T., Tauler J., Borowicz S., Lussier Y.A., Parr B.A., Cool C.D, & Winn R.A. (2015). Wnt7a is a novel inducer of β-catenin-independent tumor-suppressive cellular senescence in lung cancer. Oncogene, 34(42), 5317-5328.

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Protein Extraction and Western Blot Analysis

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Lung tissue protein extracts were obtained from mouse lungs homogenized in tissue protein extraction lysis buffer containing a cocktail of 1M HEPES, 5M NaCl, 10% Triton X-100, 1M DTT, 0.5% EDTA, 20mM NaVO₃, 10mM PMSF, 0.5M Na-β-glycerophosphate and protease inhibitors using Qiagen TissueLyser LT (Qiagen, Venlo, Limburg), while NSCLC cells were lysed in a lysis buffer (MAP kinase lysis buffer) and the western blot analysis was carried out as previously described (18 (link), 19 (link)). Anti-β-actin (A3853) and anti-β-catenin (C2206) were purchased from SIGMA (Sigma-Aldrich, St. Louis, MO). Anti-Caspase 3 (9662S), anti-Skp2 (4313S), anti-p27^kip (2552S), anti-Cdk6 (3136S), anti-CyclinD1 (2922S), anti-LC3A/B (4108S) anti-p-pRB (3590S) were purchased from Cell signaling (Cell signaling Technology Inc, Danvers, MA) and anti-Wnt7a (SC26360) was purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Santa Cruz, CA). Densitometric analysis was performed using ImageJ software. Band intensities were normalized to their corresponding controls and are represented in the figures.

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Quantification of Intestinal Polyp Subpopulations

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Dissected intestinal tissues were fixed with 4% paraformaldehyde, embedded in paraffin wax and sectioned at a thickness of 4 µm at 40 µm intervals throughout the tissue block and stained with anti-β-catenin (c2206; Sigma-Aldrich) antibodies. Polyps were categorized as β-catenin-positive or β-catenin-negative and scored for numbers.

Biyajima K., Kakizaki F., Shen X., Mori K., Sugai M., Taketo M.M, & Yokota Y. (2015). Id2 deletion attenuates Apc-deficient ileal tumor formation. Biology Open, 4(8), 993-1001.

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Phosphoprotein Profiling of HK-2 Cells

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Total cellular protein (3 mg) from HK‐2 cells were incubated with an anti‐Dpr1 antibody (ABCAm; ab51260) and Protein G Plus Agarose (Santa Cruz Biotechnology; sc‐500778) at 4°C overnight. After washing, Ser/Thr‐phosphorylated proteins were isolated using a phosphorylation purification kit according to the manufacturer's instructions (Qiagen, Life Technologies; 37145). The purified protein concentration was adjusted to 0.1 mg/mL; a 30‐µL aliquot was used for Western blotting. Western blotting was performed using an anti‐phosphoserine/threonine antibody (Sigma‐Aldrich; P3430).
For Western blotting, protein concentrations were determined using BCA (Life Technologies; 23227). The anti–E‐cadherin (#3195), anti–N‐cadherin (#13116), anti‐cyclin D1 (#2978), anti–β‐tubulin (#86298), anti–phospho‐β‐catenin (Ser33/37/Thr41) (#9561) and anti‐Dvl2 (#3224) antibodies were from Cell Signaling Technology. The anti‐collagen IV (ab6586) antibody was from ABCAm. The anti‐GSK3β (sc‐71186) and anti–phospho‐GSK3β (Ser9) (sc‐11757) antibodies were from Santa Cruz Biotechnology. The anti–β‐catenin (C2206) and anti‐cyclin G2 (HPA034684) antibodies were from Sigma‐Aldrich, and the anti‐MMP7 (10374‐2‐AP) antibody was from Proteintech. All experiments were performed in triplicate.

Zhao C., Gao J., Li S., Liu Q., Hou X., Xing X., Wang D., Sun M., Wang S, & Luo Y. (2020). Cyclin G2 regulates canonical Wnt signalling via interaction with Dapper1 to attenuate tubulointerstitial fibrosis in diabetic nephropathy. Journal of Cellular and Molecular Medicine, 24(5), 2749-2760.

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