Creb 48h2 rabbit mab

SDS-PAGE and Western blot analyses used the following primary antibodies. The reents were purchased as follows: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (14C10) Rabbit mAb (cat. #2118, Cell Signaling, Beverly, MA, USA), Phospho-p44/42MAPK (Erk1/2) Rabbit mAb (cat. #4370, Cell Signaling, USA), p44/42 MAPK (Erk1/2) Rabbit mAb (cat. #4695, Cell Signaling, USA), CREB (48H2) Rabbit mAb (cat. #9197, Cell Signaling, USA), Purified Mouse Anti-Arc (cat. #612602, San Jose, CA, USA), and Rabbit monoclonal [E113] to CREB (phospho S133; cat. #ab32096, Abcam, Cambridge, MA, USA).
The results were scanned into the computer, and we used Image J software to analyze the gray value of the protein strip.

The total amount of crude drugs in HJSD is 60 g, among which the drug composition and dose are Scutellaria baicalensis (Huang qin) 12 g, Gynostemma pentaphylla (Jiao gu lan) 12 g, Radix bupleuri (Chai hu) 12 g, Rhizoma acori graminei (Shi chang pu) 9 g, lotus petiole (He geng) 9 g, and basil (Luo le) 6 g.
The HJSD and sodium valproate tablets (VPA, Hunan Xiangzhong Pharmaceutical Co. Ltd.) were purchased from the Affiliated Hospital of Hebei Academy of Chinese Medicine Sciences. Pentylenetetrazol (PTZ, P6500-25G, Sigma-Aldrich), anti-AC rabbit polyclonal antibody (TA322232, Origene), rabbit anti-cAMP polyclonal antibody (TA327118, Origene), CREB (48H2) Rabbit mAb (#9197, Cell Signaling Technology), and anti-NPY antibody (ab30914, Abcam) were purchased from Hebei Bio-high Technology Co. Ltd., China. All other chemicals were sourced from the Hebei Academy of Chinese Medicine Sciences and were of analytical grade.