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Hif 1α

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HIF-1α is a protein subunit that is a component of the hypoxia-inducible factor-1 (HIF-1) transcription complex. HIF-1 is a key regulator of cellular and physiological responses to low oxygen levels (hypoxia).

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Lab products found in correlation

Accupower rt premix, by Bioneer (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Cfx connect real time pcr detection system, by Bio-Rad (1 mentions) Dnase 1, by New England Biolabs (1 mentions) Rotor gene 6000 series software, by Qiagen (1 mentions) Vascular endothelial growth factor (vegf), by Bioneer (1 mentions) Rt2 sybr green qpcr mastermix, by Qiagen (1 mentions) Glut1, by Bioneer (1 mentions)

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HIF-1α-siRNA (2 citations)

2 protocols using hif 1α

1

Quantification of Hypoxia-Responsive Genes

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Total RNA was extracted from RKO and HCT116 cells using the TRIzol reagent (Invitrogen) and treated with DNase I (New England Biolabs). cDNA was synthesized from (1 μg) of total RNA using AccuPower RT PreMix (Bioneer, Daejeon, Republic of Korea), and then amplified by PCR using the appropriate primers for Glut1 (glucose transporter 1), PDK1 (pyruvate dehydrogenase kinase 1), PGK1 (phosphoglycerate kinase 1), CA9 (carbonic anhydrase 9), HIF-1α, and 18S rDNA (Bioneer). qPCR and analyses were performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad).
Oh E.T., Kim C.W., Kim H.G., Lee J.S, & Park H.J. (2017). Brusatol-Mediated Inhibition of c-Myc Increases HIF-1α Degradation and Causes Cell Death in Colorectal Cancer under Hypoxia. Theranostics, 7(14), 3415-3431.
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2

Hypoxia Pathway Gene Expression

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Real-time PCR was performed using RT2 SYBR Green qPCR Mastermix (Qiagen, Venlo, The Netherlands). Data were analyzed using the Rotor-Gene 6000 Series Software (Corbett Research, Cambridge, UK). VEGF, EPO, GLUT1, PDK1, and HIF-1α primers were purchased from Bioneer (Daejeon, Korea).
Ban H.S., Kim B.K., Lee H., Kim H.M., Harmalkar D., Nam M., Park S.K., Lee K., Park J.T., Kim I., Lee K., Hwang G.S, & Won M. (2017). The novel hypoxia-inducible factor-1α inhibitor IDF-11774 regulates cancer metabolism, thereby suppressing tumor growth. Cell Death & Disease, 8(6), e2843-.
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