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Ultrasensitive mouse insulin kit

Manufactured by ALPCO
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The Ultrasensitive Mouse Insulin kit is a laboratory equipment designed to measure insulin levels in mouse samples. The kit utilizes an immunoassay technique to quantify insulin concentrations accurately and sensitively.

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Lab products found in correlation

Au680 chemistry analyzer, by Beckman Coulter (1 mentions)

Spelling variants of this product

Insulin (40 citations) Mouse insulin kit (2 citations)

4 protocols using ultrasensitive mouse insulin kit

1

Measuring Glycemic and Insulin Levels

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Blood samples were collected to measure blood glucose and serum insulin. The blood sampling line was filled with a solution of 4.5% EDTA to prevent blood clotting. Samples were kept on ice, and plasma was isolated and stored at −70°C until analysis. Glucose levels were measured with a glucose analyzer (OneTouch Ultra). Insulin levels were determined in duplicate using 5 μL of serum and an Ultrasensitive Mouse Insulin kit (ALPCO, Windham, NH) according to the manufacturer's instructions.
Seo E., Lee E.K., Lee C.S., Chun K.H., Lee M.Y, & Jun H.S. (2014). Psoralea corylifolia L. Seed Extract Ameliorates Streptozotocin-Induced Diabetes in Mice by Inhibition of Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2014, 897296.
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2

Measuring Metabolic Biomarkers in Mice

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Blood samples were collected before sacrifice to measure blood glucose and serum insulin. The blood sampling line was filled with a solution of 4.5% ethylenediaminetetraacetic acid (EDTA) to prevent blood clotting. Samples were kept on ice, and serum was isolated and stored at −70 °C until analysis. Glucose levels were measured with a glucose analyzer (OneTouch® Ultra). Insulin levels were determined in duplicate using 5 μL of serum and an UltraSensitive Mouse Insulin kit (ALPCO, Windham, NH, USA) according to the manufacturer’s instructions. Serum levels of alanine cholesterol, triglycerides, low-density lipoprotein (LDL)-cholesterol and high-density lipoprotein (HDL)-cholesterol were measured using Beckman Coulter AU680 chemistry analyzer (Beckman Coulter, Inc., Brea, CA, USA).
Seo E., Kim S., Lee S.J., Oh B.C, & Jun H.S. (2015). Ginseng Berry Extract Supplementation Improves Age-Related Decline of Insulin Signaling in Mice. Nutrients, 7(4), 3038-3053.
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3

Ultrasensitive Mouse Insulin Measurement

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Insulin concentrations were determined using the Mouse Ultrasensitive Insulin kit (Alpco Diagnostics, cat. 80-INSMSU-E01) according to the manufacturer's instructions.
Carlos D., Pérez M.M., Leite J.A., Rocha F.A., Martins L.M., Pereira C.A., Fraga-Silva T.F., Pucci T.A., Ramos S.G., Câmara N.O., Bonato V.L., Tostes R.C, & Silva J.S. (2020). NOD2 Deficiency Promotes Intestinal CD4+ T Lymphocyte Imbalance, Metainflammation, and Aggravates Type 2 Diabetes in Murine Model. Frontiers in Immunology, 11, 1265.
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4

Assessing Insulin Resistance in Plasma Samples

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Plasma samples collected at day 28 were stored at −80°C
until assayed. Measurements were made by enzyme-immunolinked assay, using the
Mouse Ultrasensitive Insulin kit (Alpco Diagnostics, Salem, NH), as previously
described[8 (link)]. Homeostasis model
assessment-insulin resistance (HOMA-IR) was calculated to assess changes in
insulin resistance (fasting insulin (mU/L) × fasting glucose (mg/dL) /
405) [21 (link)].
Holst B.D., Vanderklish P.W., Krushel L.A., Zhou W., Langdon R.B., McWhirter J.R., Edelman G.M, & Crossin K.L. (1998). Allosteric modulation of AMPA-type glutamate receptors increases activity of the promoter for the neural cell adhesion molecule, N-CAM. Proceedings of the National Academy of Sciences of the United States of America, 95(5), 2597-2602.
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