Cellquest pro analysis software

Manufactured by BD

Sourced in United States

The BD CellQuest™ Pro Analysis software is a data acquisition and analysis tool for flow cytometry. It provides users with the capability to collect, display, and analyze data from flow cytometry experiments.

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Lab products found in correlation

Facscalibur, by BD (9 mentions) Facscalibur flow cytometer, by BD (7 mentions) Fitc annexin 5 apoptosis detection kit, by BD (3 mentions) Flow cytometry tube, by BD (2 mentions) Propidium iodide, by BD (2 mentions) Annexin 5, by BD (2 mentions) Facscan flow cytometer, by BD (2 mentions) Annexin 5 fitc apoptosis detection kit, by Keygen Biotech (2 mentions) Cd43 fitc, by BD (1 mentions) Cd45 pe, by BD (1 mentions) Cd34 apc, by BD (1 mentions) 7 aminoactinomycin d 7 aad, by Merck Group (1 mentions) Facs caliber flow cytometer, by BD (1 mentions) H2dcfda, by Thermo Fisher Scientific (1 mentions) Pi flow cytometry kit, by Abcam (1 mentions) Facscalibur cytometer, by BD (1 mentions) Cell cycle kit, by Keygen Biotech (1 mentions) Facscalibur system, by BD (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Fitc anti human cd127, by BD (1 mentions)

27 protocols using cellquest pro analysis software

Cell Cycle and Death Analysis by Flow Cytometry

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Cell cycle analysis and determination of cell death were carried out by propidium iodide staining and flow cytometry essentially as previously described^{22 (link)}. The analysis of cells was carried out with a FACSCalibur flow cytometer (BD Biosciences). Acquired data were processed by Cell Quest Pro Analysis software (BD Biosciences). For each measurement, 10,000 events were analyzed. Flow cytometry of trypsin-digested mouse embryo cells was performed as described in^{48 (link)}. Cells were analyzed using a FACScan flow cytometer (BD Biosciences) and data were processed using Cell Quest Pro Analysis software (BD Biosciences).

Schaefer S., Doktor T.K., Frederiksen S.B., Chea K., Hlavacova M., Bruun G.H., Rabjerg M., Andresen B.S., Dominguez I, & Guerra B. (2019). Down-regulation of CK2α correlates with decreased expression levels of DNA replication minichromosome maintenance protein complex (MCM) genes. Scientific Reports, 9, 14581.

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Immunophenotyping of Hematopoietic Cells

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EB were collected as previously described, the resulting single-cell suspension was incubated with the fluorochrome-conjugated antibodies (mAbs): CD34-APC, CD43-FITC and CD45-PE (antibodies from BD Biosciences).
Cells were incubated with selected antibodies for 30 minutes on ice, washed and resuspended in PBS containing 5% FCS and 7-aminoactinomycin D (7AAD; 1μg/mL) (Sigma Aldrich) to exclude dead cells. Isotype matched FITC-, PE- and APC-conjugated irrelevant mAbs (BD Biosciences and Beckman Coulter). Analysis was carried out with a FACSCalibur flow cytometer using CellQuest Pro Analysis software (Becton-Dickinson).

Féraud O., Valogne Y., Melkus M.W., Zhang Y., Oudrhiri N., Haddad R., Daury A., Rocher C., Larbi A., Duquesnoy P., Divers D., Gobbo E., Brunet de la Grange P., Louache F., Bennaceur-Griscelli A, & Mitjavila-Garcia M.T. (2016). Donor Dependent Variations in Hematopoietic Differentiation among Embryonic and Induced Pluripotent Stem Cell Lines. PLoS ONE, 11(3), e0149291.

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Apoptosis Induction by DMHE in HT-29 Cells

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Approximately 1 × 10⁶ HT-29 cells were incubated in petri dishes for 24 h. Various concentrations of DMHE (25, 50 and 75 μg/mL) were then added to the cells and incubated for 24, 48 and 72 h, respectively. The cells were harvested with accutase in phosphate buffered saline by centrifugation, stained with annexin V-FITC only, propidium iodide only and double stained with annexin V-FITC/PI according to the Annexin V-FITC Apoptosis Detection Kit protocol (BD Pharmingen™, BD Bioscience, San Jose, CA, USA). The stained cells were examined using a FACScalibur flow cytometer (BD Bioscience) and the data analyzed using CellQuest Pro analysis software (Becton Dickinson, San Jose, CA, USA). A total of 10,000 cells were counted for each treatment, and the apoptotic and total death rates were determined.

Lay M.M., Karsani S.A, & Abd Malek S.N. (2014). 1-(2,6-Dihydroxy-4-methoxyphenyl)-2-(4-hydroxyphenyl) Ethanone-Induced Cell Cycle Arrest in G1/G0 in HT-29 Cells Human Colon Adenocarcinoma Cells. International Journal of Molecular Sciences, 15(1), 468-483.

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Apoptosis Quantification in HT-29 Cells

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The quantification of cell death was determined by flow cytometry using the Annexin V-FITC apoptosis detection kit according to the manufacturer's instructions (BD Pharmingen, BD Bioscience, USA). Briefly, 1 × 10⁶ of the HT-29 cells were seeded into each Petri dish (30 mm) and after a 24 h incubation, various concentrations of the test compound were added and incubated for 24 h, 48 h, and 72 h, respectively. The cells were then washed with PBS, suspended in annexin V binding buffer and then added to an annexin V-FITC solution and propidium iodide (PI) for 10 minutes at room temperature. The samples were then analyzed using FACScalibur (BD Bioscience, USA) using CellQuest Pro analysis software (Becton Dickinson, USA).

Lay M.M., Karsani S.A, & Abd Malek S.N. (2014). Induction of Apoptosis of 2,4′,6-Trihydroxybenzophenone in HT-29 Colon Carcinoma Cell Line. BioMed Research International, 2014, 468157.

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Cell Cycle Analysis of DT40 Cells

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DT40 cells (2 × 10⁶ cells/ml) were incubated in tissue culture flasks with varying concentrations (0.02, 0.2, 2, and 20 µg/ml) of BP5 in RPMI 1640 medium (final volume, 10 ml) containing 1% FBS plus 1% CHS for 72 h. The cells were collected 24, 48, and 72 h post-seeding, washed twice with cold phosphate-buffered saline (PBS), resuspended in 70% cold ethanol, and incubated at 4°C for 2 h. Then, the cells were washed with cold PBS and incubated in 0.5 ml of propidium iodide (PI)-binding buffer (50 µg/ml of PI and 100 µg of ribonuclease) at 37°C for 30 min. The cell cycle distribution of DT40 cells was determined on a FACSCaliber flow cytometer with CellQuest Pro analysis software (Becton-Dickinson, Brea, CA, USA). The experiments were performed in triplicate.

Ji X.B., Luo J., Feng X.L., Xu Q.L., Man T., Zhao D., Li X.F., Zhang G.P, & Chen P.Y. (2018). The immunomodulatory peptide bursopentin (BP5) enhances proliferation and induces sIgM expression in DT40 cells. African Health Sciences, 18(4), 1292-1302.

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Measuring Intracellular ROS with H2DCFDA

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The intracellular production of reactive oxygen species (ROS) was measured using H₂DCFDA (D399, Life Technologies, Carlsbad, USA). Cells were incubated with 100 nM 1,25(OH)₂D₃ for 24 h followed by exposure to 1 mM hydrogen peroxide for 1 h or 24 h (Scheme 1B). Thirty minutes before the end of the incubation, H₂DCFDA was aded to a final concentration of 10 μM. Cells were washed and suspended in cold PBS. Samples were kept on ice and analyzed using the FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, USA) using CellQuest Pro analysis software (Becton Dickinson, Franklin Lakes, USA).

Anna P., Justyna W., Tomasz Ś., Michał W., Robert C.T., Andrzej T.S, & Michał A.Ż. (2016). Vitamin D derivatives enhance cytotoxic effects of H2O2 or cisplatin on human keratinocytes. Steroids, 110, 49-61.

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Cell Cycle Analysis by Flow Cytometry

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Cell cycle distribution analysis was performed using the Propidium Iodide (PI) Flow Cytometry Kit (ab139418, Abcam, Cambridge, UK) following the manufacturer’s protocol. Untreated cells were used as control. Cells were prepared at a density of 1x10⁴ per well in 6 well plates and exposed to test reagents for 24 h at 37ºC. After harvesting and preparation of single-cell suspensions, cells were fixed, stained with PI, and analyzed on a FACSCalibur cytometer (BD Biosciences, San Jose, CA, US). Cell cycle distribution analysis was performed on three separate experiments using BD CellQuest™ Pro Analysis software (BD Biosciences, San Jose, CA, US).

Shan L., Liu W, & Zhan Y. (2019). Sulfated polysaccharide of Sepiella maindroni ink targets Akt and overcomes resistance to the FGFR inhibitor AZD4547 in bladder cancer. Aging (Albany NY), 11(18), 7780-7795.

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Cell Cycle Analysis by PI Staining

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Cells were stained with propidium iodide (PI) using the Abcam kit (ab139418) according to the provided protocol. Briefly, cells were harvested, washed in ice-cold phosphate-buffered saline (PBS) and fixed in 70% ethanol for 30 minutes at 4°C. After two PBS washes, cells were treated with RNase A for 15 minutes at 37°C, stained with 5 μg/mL PI in PBS and assayed with a FACS Calibur (BD Biosciences, San Jose, CA, USA) flow cytometer using Cell Quest software. The cell cycle distribution was analyzed using BD CellQuest™ Pro Analysis software (BD Biosciences).

Raha P., Thomas S., Thurn K.T., Park J, & Munster P.N. (2015). Combined histone deacetylase inhibition and tamoxifen induces apoptosis in tamoxifen-resistant breast cancer models, by reversing Bcl-2 overexpression. Breast Cancer Research : BCR, 17(1), 26.

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Cell Cycle Analysis by Flow Cytometry

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Cells were stained with propidium iodide (PI) using the cell cycle kit (#KGA511, KeyGEN BioTECH, Nanjing, China) according to the provided protocol. Briefly, cells were harvested, washed in ice-cold phosphate-buffered saline (PBS), and fixed in 70% cold ethanol for 2h at 4°C. After two PBS washes, cells were treated with RNase A/PI staining buffer and assayed with an FACS Calibur (BD Biosciences, San Jose, CA, USA) flow cytometer using Cell Quest software. The cell cycle distribution was analyzed using BD CellQuest™ Pro Analysis software (BD Biosciences, San Jose, CA, USA).

Liu J., Miao X., Xiao B., Huang J., Tao X., Zhang J., Zhao H., Pan Y., Wang H., Gao G, & Xiao G.G. (2020). Obg-Like ATPase 1 Enhances Chemoresistance of Breast Cancer via Activation of TGF-β/Smad Axis Cascades. Frontiers in Pharmacology, 11, 666.

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Quantifying Platelet Receptor Occupancy

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PAC-1 occupied by RUC-4 [30 (link)].
As a result, PAC-1 binding to activated platelets identifies the receptors
that are not already bound by RUC-4, providing information on the percentage
of receptors occupied by RUC-4. In the assay, 25 µl of whole blood
from 5 separate donors (3 female and 2 male) was incubated for 5 minutes at
room temperature with 12.5 µl of FITC-labeled 25 µg/ml PAC-1
(final concentration: 6.25 µg/ML), either 0.4 µl of 1 mM PGE-1
(negative control) or 0.4 µl of varying concentrations of RUC-4
(final concentrations 3 µM, 1 µM, 0.5 µM, 0.3
µM, 0.1 µM, 0.03 µM and 0.01 µM), and 27
µl of 1X HBMT with 1 mM Mg²⁺. 5 µl of 200 µM
ADP (final concentration 20 µM) was added to the above mixture and
incubated for 30 minutes at room temperature. 450 µl of 1X HBMT with
1 mM Mg²⁺ was added to make up the final volume to 500 µl
and flow cytometry was conducted immediately at room temperature using the
FACSCalibur (Becton Dickinson, BD).
Flow cytometry data were analyzed using BD CellQuest™ Pro
Analysis software. Data were expressed as both the geometric mean
fluorescence and the percentage of the mean geometric fluorescence obtained
in the absence of RUC-4.

Vootukuri S., Li J., Nedelman M., Thomas C., Jiang J.K., Babayeva M, & Coller B.S. (2019). Preclinical studies of RUC-4, a novel platelet αIIbβ3 antagonist, in non-human primates and with human platelets. Journal of Clinical and Translational Science, 3(2-3), 65-74.

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