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2 protocols using labostar

1

Preparation of Thymine Crystals

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The thymine crystals were prepared by depositing and drying 20 µL of 1 mg/mL solution of thymine28 (T0376, Sigma-Aldrich) in ultra-pure water (Labostar, Siemens) on an untreated glass coverslip (Menzel Glaser, Braunschweig, Germany). The sample preparation was carried out under ambient conditions: at room temperature and relative humidity of about 40%. The results of the sample preparation procedure were well reproducible.
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2

Electrochemical Sensing Platform Fabrication

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Graphite powder (≤20 µM), α-Al2O3, potassium permanganate, sodium nitrate, hydrogen peroxide (H2O2), LiClO4, potassium chloride, sodium hydroxide, ruthenium hexamine chloride, dopamine, p-aminothiophenol, p-aminophenol, p-phenylenediamine, p-aminobenzoic acid and sulfanilic acid were purchased from Sigma-Aldrich. Sulfuric acid was purchased from Fisher-Scientific. Potassium ferrocyanide and potassium hydrogen phosphate were purchased from Hi-Media. Acetonitrile was purchased from SRL. All the chemicals were used as received without any purification. Ultrapure water from Siemens LaboStar (resistivity ~18.2 MΩ.cm) system was used to prepare phosphate buffer and other aqueous solutions.
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