The paired primary tumor and lymph node metastasis samples were sectioned at 4 μm and placed on the same slide. After de-paraffinization, hydration, and antigen retrieval using boiling, slides were stained using primary antibody against EGFR (RMA-0804, MXB Biotechnologies), HER2 (ab16662, Abcam), or HER3 (ab93739, Abcam). After staining with secondary antibody (ab6721, Abcam), slides were counterstained with hematoxylin and visualized using 3,3′-diaminobenzidine (DAB). Tumor samples which were stained positive in previous tests were used as positive control, and negative controls were prepared by replacing primary antibody with phosphate-buffered saline.
Ab93739
Manufactured by Abcam
Sourced in United Kingdom
Ab93739 is a lab equipment product. It is a microscope slide designed for use in microscopy and imaging applications. The product provides a standard glass slide format for mounting and analyzing samples.
Automatically generated - may contain errors
2 protocols using ab93739
1
Immunohistochemical Evaluation of Tumor Markers
2
Immunohistochemical Analysis of HER2/neu, HER3, and NRG1
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Immunohistochemistry was performed using 5 μm whole standard tissue sections of FFPE tumour samples. For the detection of HER2/neu, a prediluted anti-HER2/neu rabbit monoclonal antibody (clone 4B5; Ventana Medical Systems, Oro Valley, Arizona, USA) was used as the primary antibody. The staining was performed on a Ventana Benchmark XT autostainer using the XT UltraView diaminobenzidine kit (Ventana Medical Systems) following the manufacturer's protocols. Staining of HER3 and NRG1 was performed using the Vectastain ABC-Kit Elite Universal detection system (Vector Laboratories, Peterborough, UK). For HER3 immunohistochemistry, a monoclonal rabbit antibody was used as the primary antibody (ab93739; Abcam, Cambridge, UK). NRG1 staining was performed using a polyclonal human antibody (HPA010964; Atlas Antibodies, Stockholm, Sweden).
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