Ammonium persulfate, TEMED, sodium dodecyl sulfate (SDS), acrylamide, and N,N´‐methylenebisacrylamide were purchased from SERVA (Heidelberg, Germany). Human uPA and Glu‐type plasminogen (Glu‐Plg) were products of Calbiochem (Darmstadt, Germany). BSA was from Carl Roth (Karlsruhe, Germany). Beriglobin was from CSL Behring (King of Prussia, PA, USA). Polybrene, puromycin, and tranexamic acid (TA) were from Sigma–Aldrich (St. Louis, MO). Nonidet P‐40 was obtained from Pierce Biotechnology Inc. (Rockford, IL, USA). Annexin V‐Pacific blue was from BioLegend (San Diego, CA, USA).
N n methylenebisacrylamide
Manufactured by Serva Electrophoresis
Sourced in Germany, United States
N,N′-methylenebisacrylamide is a chemical used as a cross-linking agent in polyacrylamide gel electrophoresis. It functions by forming covalent bonds between acrylamide polymer chains, creating a three-dimensional network structure within the gel.
Automatically generated - may contain errors
Lab products found in correlation
Acrylamide, by Serva Electrophoresis (3 mentions)
Asolectin, by Merck Group (2 mentions)
Celite 535, by Carl Roth (2 mentions)
Hydroxylapatite bio gel htp, by Bio-Rad (2 mentions)
Amberlite bio beads sm 2, by Bio-Rad (2 mentions)
Triton x 114, by Serva Electrophoresis (2 mentions)
14c 2 oxoglutarate, by PerkinElmer (2 mentions)
14c malate, by PerkinElmer (2 mentions)
Bovine serum albumin (bsa), by Carl Roth (1 mentions)
Ammonium persulfate, by Serva Electrophoresis (1 mentions)
Beriglobin, by CSL Behring (1 mentions)
Annexin 5 pacific blue, by BioLegend (1 mentions)
Polybrene, by Merck Group (1 mentions)
Puromycin, by Merck Group (1 mentions)
Triton x 100, by Serva Electrophoresis (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Cardiolipin, by Merck Group (1 mentions)
Hemin, by Merck Group (1 mentions)
Triton x 100, by Bio-Rad (1 mentions)
Bromophenol blue sodium salt, by Serva Electrophoresis (1 mentions)
4 protocols using n n methylenebisacrylamide
1
SDS-PAGE Reagent Preparation
2
Isolation and Characterization of Mitochondria
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Hydroxylapatite (Bio-gel HTP) and Amberlite Bio-Beads SM-2 was obtained from Bio-Rad; Matrex Gel Orange was obtained from Amicon (Beverly, MA, USA); Triton X-100, Triton X-114, acrylamide, and N,N′-methylenebisacrylamide were obtained from Serva; egg-yolk phospholipids were obtained from Fluka; hemin, cardiolipin, 1,4-piperazine-diethanesulphonic acid (Pipes), Sodium dodecyl sulfate, (SDS) and asolectin were obtained from Sigma; celite 535 was obtained from Roth; Sephadex G-75 was obtained from Pharmacia; [14C] 2-oxoglutarate and [14C] malate were purchased from Perkin-Elmer Life Sciences (Waltham, MA, USA). All other chemicals used were of analytical grade.
3
Purification and Analysis of Mitochondrial Enzymes
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In regards to chemicals: [14C] 2-oxoglutarate and [14C] malate were purchased from Perkin-Elmer Life Sciences; hydroxylapatite (Bio-gel HTP) and Amberlite Bio-Beads SM-2 from Bio-Rad; Triton X-100, Triton X-114, acrylamide and N,N′-methylenebisacrylamide from Serva; egg yolk phospholipids from Fluka; Matrex Gel Orange from Amicon (Beverly, MA, USA); hemin, cardiolipin, 1,4-piperazine-diethanesulphonicacid (Pipes), sodium dodecyl sulfate (SDS) and asolectin from Sigma; celite 535 from Roth and Sephadex G-75 from Pharmacia. All other chemicals used were of analytical grade.
4
Antioxidant and Protein Analysis Reagents
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Trichloroacetic acid (TCA), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) and 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) were purchased from Sigma-Aldrich (Munich, Germany). Bromophenol blue sodium salt (3′,3″,5′,5″-tetrabromophenol sulfophthalein sodium salt), 2-mercaptoethanol, acrylamide, N,N′-methylenebisacrylamide, sodium dodecylsulphate (SDS), and Coomassie Brilliant Blue R-250 were purchased from SERVA Electrophoresis GmbH (Munich, Germany). PageRuler Plus Protein Ladder 10 to 250 kDa was purchased from Thermo Fisher Scientific (Waltham, MA, USA), and 2-D Quant Kit from GE Healthcare Bio-Sciences (Marlborough, MA, USA). Fluorescein sodium salt was purchased from Fluka (Everett, WA, USA). Pronase E (4,000,000 PU/g) was purchased from Merck (Darmstadt, Germany). Other solvents and reagents of analytical (ACS) or HPLC grade were purchased from Sigma-Aldrich, SERVA Electrophoresis (Munich, Germany) or POCH (Gliwice, Poland).
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